Fig 2: Cisatracurium could not function more in siChrna1-treated mice. Mice were treated with siControl (SiCtrl) or SiChrna1 72 h before the administration of vehicle or 1 mg/kg cisatracurium (CIS) 6 h before hyperhidrosis induction. (A) The mRNA level of Chrna1 in sweat glands of hyperhidrosis mice. (B and C) The protein level of Chrna1 in sweat glands of hyperhidrosis mice. (D) The number of black dots were calculated using ImageJ after pilocarpine injection. n = 6. ### p < 0.001, ***p < 0.001, ns, not significant.

Fig 3: Overexpression of Chrna1 abolished the effect of cisatracurium on hyperhidrosis. Mice were treated with virus packaged with empty vectors (EV) or Chrna1-expressing sequences 72 h before the administration of vehicle or 1 mg/kg cisatracurium (CIS) 6 h before hyperhidrosis induction. (A) The mRNA level of Chrna1 in sweat glands of hyperhidrosis mice. (B and C) The protein level of Chrna1 in sweat glands of hyperhidrosis mice. (D) The number of black dots were calculated using ImageJ after pilocarpine injection. n = 6. ### p < 0.001, ***p < 0.001, ns, not significant.