Fig 2: Inhibition of lncRNA WDR11-AS1 promotes ECM degradation in chondrocytes. (A) Expression of lncRNA WDR11-AS1 was determined by RT-qPCR in SW1353 cells transfected with three siRNAs (50 nM) targeting endogenous WDR11-AS1. (B) Western blot analysis showed COLII, ACAN, MMP3, MMP13, ADAMTS4, and ADAMTS5 protein levels in SW1353 cells after WDR11-AS1 knockdown. (C) Western blot analysis showed COLII, MMP3, and ADAMTS5 protein levels in TNF-a (10 ng/mL)-stimulated SW1353 cells after WDR11-AS1 knockdown. (D,E) Western blot analysis showed COLII, ACAN (D), MMP13, and ADAMTS5 (E) protein levels in IL-1ß or TNF-a (10 ng/mL)-stimulated primary chondrocytes after WDR11-AS1 knockdown. * p < 0.05, si-RNA vs. si-NC (A,B); the measurement data are expressed as the mean ± standard deviation, and were analyzed by Mann-Whitney U test. The experiments were repeated three times.