Fig 1: Cell type dependent regulation of SLC7A11, ALOX5, and ATF4 by E2F1.(A) Western blot analysis of the effect of E2F1 knock-down in HT1080 and NARF2 cells. Three days after transfection, protein levels of E2F1, SLC7A11, ALOX5, ATF4 were determined using ß-actin as a loading control. (B) Bar graph shows the fold of protein levels of HT1080 cells in siE2F1 vs control siNeg. Transcripts level of the indicated genes were measured in both HT1080 (C) or NARF2 (D) cells after E2F1 or scrambled (siNeg) knock down for 3 days. Original western blots are shown in S. Figure 9. (* p < 0.05)