Fig 2: Correlation between miRNA targeted-genes expression and immune infiltration. A Correlation of CD320 expression with immune infiltration level in HCC. B Correlation of PSMD14 expression with immune infiltration level in HCC. C Correlation of NTF3 expression with immune infiltration level in HCC. D Correlation of SORT1 expression with immune infiltration level in HCC. E The correlation between the four hub genes (CD320, PSMD14, SORT1, and NTF3) and the cell markers of corresponding immune cells by Cell Marker database

Fig 3: Gene set enrichment analysis of miRNA targeted-genes in HCC tissues. A–D The GSEA analysis of CD320, PSMD14, NTF3, and SORT1

Fig 4: Survival Analysis and Prognostic Model. A The ROC curve of CD320, PSMD14, NTF3, and SORT1 in HCC. B The univariate cox regression analysis of CD320, PSMD14, NTF3, SORT1 and clinicopathological characteristics. C The multivariate cox regression analysis of CD320, PSMD14, NTF3, SORT1. D The Nomogram shows the prognostic model of CD320, PSMD14, NTF3, SORT1

Fig 5: Validation of miRNA and their targeted-genes expressions. A-a, b and B-a–d The qRT-PCR results of hsa-miR-125b-5p, hsa-miR-21-5p, CD320, PSMD14, SORT1, and NTF3 in different cell lines. C-a–d The qRT-PCR results of CD320, NTF3, PSMD14 and SORT1 in 15 pairs of human liver cancer and para-cancerous tissues. D Western blot of CD320, NTF3, PSMD14 and SORT1 in 12 pairs HCC tumor tissues and matched adjacent normal tissues. E-a–d IHC of CD320, NTF3, PSMD14 and SORT1 in 15 pairs HCC tumor tissues and matched adjacent normal tissues. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not statistically significant)