MOUSE ANTI INFLUENZA A NUCLEOPROTEIN MCA400 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemMOUSE ANTI INFLUENZA A NUCLEOPROTEIN
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMCA400
CloneAA5H
ImmunogenInfluenza A / Puerto Rico / 8 / 34 (H1N1) and A/Bangkok / 1 / 79 (H3N2) viruses
TargetINFLUENZA A NUCLEOPROTEIN
FormatPurified IgG - liquid
HostMouse
ApplicationsWestern Blotting,Immunohistology - Paraffin,Immunofluorescence
IsotypeIgG2a
Quantity1 mg
ReactivityViral
ClonalityMonoclonal Antibody
ConcentrationIgG concentration 1.0 mg/ml

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Infection Studies with Airway Organoids from Carollia perspicillata Indicate That the Respiratory Epithelium Is Not a Barrier for Interspecies Transmission of Influenza VirusesMicrobiology SpectrumApril 13, 2023Ang Su, Miaomiao Yan, Suvarin Pavasutthipaisit, Kathrin D. Wicke, Guntram A. Grassl, Andreas Beineke, Felix Felmy, Sabine Schmidt, Karl-Heinz Esser, Paul Becher, et al.mouse, non-human primate, pig, rabbit, rat, sheep, tag, virus, Xenopus, zebrafish" IAV virus NP,Bio-Rad,MCA400,Virus Alexa Fluor 488 goat anti mouse,Thermo Fisher Scientific,A-11001,Mouse Alexa Fluor 568 goat

(2) RSV-induced expanded ciliated cells contribute to bronchial wall thickeningVirus ResearchApril 2, 2023Sattya N. Talukdar, Jaspreet Osan, Ken Ryan, Bryon Grove, Danielle Perley, Bony D. Kumar, Shirley Yang, Sydney Dallman, Lauren Hollingsworth, Kristina L. Bailey, et al.monoclonal (1:25) (MCA4674, Bio-Rad), or anti-Influenza A nucleoprotein (NP) mouse monoclonal (1:30) (MCA400, Bio-Rad). The next day, after washing with the wash buffer, the inserts were incubated with an anti-rabbit

(3) Bacterial vesicles block viral replication in macrophages via TLR4-TRIF-axisCell communication and signaling : CCSMarch 28, 2023Bierwagen J, Wiegand M, Laakmann K, Danov O, Limburg H, Herbel SM, Heimerl T, Dorna J, Jonigk D, Preußer C, et al.virus-infected cells were stained with primary antibody (1:2,000) (mouse anti-influenza A nucleoprotein, MCA400, Bio-Rad, Germany) and the secondary antibody (1:4,000) (Goat Anti-Mouse IgG-HRP, 170–6516, Bio-Rad,

(4) Surfactant protein A alters endosomal trafficking of influenza A virus in macrophagesFrontiers in ImmunologyMarch 7, 2023Eric Yau, Linlin Yang, Yan Chen, Todd M. Umstead, Hannah Atkins, Zoe E. Katz, Jonathan W. Yewdell, Chintan K. Gandhi, E. Scott Halstead, Zissis C. ChroneosBlots were probed at 4°C overnight with NS1 (Invitrogen Cat No PA532243, 1:1000), NP (Bio-Rad Cat No MCA400, 1:1000), HA (InVitrogen Cat No MA5-29931, 1:2000), or in-house rabbit anti-human SP-A antibody (1:1000),

(5) Influenza A virus activates cellular Tropomyosin receptor kinase A (TrkA) signaling to promote viral replication and lung inflammationPLOS PathogensSeptember 19, 2022Vikram Verma, Mythili Dileepan, Qinfeng Huang, Thu Phan, Wei-Shou Hu, Hinh Ly, Yuying Liangconducted in the presence of trypsin at a concentration of 2.5 μg per ml. Anti-influenza NP mouse antibody (MCA400) was purchased from Bio-Rad (Hercules, CA). Anti-TrkA rabbit antibody (Cat#2505) and anti-phospho-TrkA

(6) Functional and Binding H1N1pdm09-Specific Antibody Responses in Occasionally and Repeatedly Vaccinated Healthcare Workers: A Five-Year Study (2009-2014)Frontiers in ImmunologyDecember 6, 2021Håkon Amdam, Anders Madsen, Fan Zhou, Amit Bansal, Mai-Chi Trieu, Rebecca Jane Cox32213) (0.6% H2O2) in 20 minutes. Mouse anti-influenza A nucleoprotein antibodies (AbD Serotec, USA, MCA400) was added, and the plates were incubated for one hour at 37°C. HRP secondary antibody (Dako, Denmark,

(7) Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophagesImmunobiologyNovember 1, 2021Yau E, Chen Y, Song C, Webb J, Carillo M, Kawasawa YI, Tang Z, Takahashi Y, Umstead TM, Dovat S, et al.Blocked cells were probed using Rab5 (Cell Signaling Technology Cat#3547S, 1:200) and NP (BioRad Cat#MCA400, 1:1000) overnight at 4°C, and labeled using AF488 conjugated anti-mouse IgG (Jackson ImmunoResearch

(8) A prenylated dsRNA sensor protects against severe COVID-19Science (New York, N.Y.)October 29, 2021Wickenhagen A, Sugrue E, Lytras S, Kuchi S, Noerenberg M, Turnbull ML, Loney C, Herder V, Allan J, Jarmson I, et al.achieved using the mouse anti–influenza A virus nucleoprotein monoclonal antibody clone AA5H (BioRad, MCA400) and visualized with goat anti-mouse IgG (H+L)–horseradish peroxidase conjugate (BioRad, 1721011) and

(9) Cigarette Smoke Affects Dendritic Cell Populations, Epithelial Barrier Function, and the Immune Response to Viral Infection With H1N1Frontiers in MedicineNovember 6, 2020Olga Danov et al.repeated and the cells were stained with primary antibody (1:2,000) (mouse anti-influenza A nucleoprotein, MCA400, Bio-Rad, Germany) diluted in 5% milk/PBS at 37°C for 1 h. Subsequently the cells were washed with 0.02%

(10) Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during InfectionCellJune 25, 2020Ho JSY, Angel M, Ma Y, Sloan E, Wang G, Martinez-Romero C, Alenquer M, Roudko V, Chung L, Zheng S, et al.in 1% Triton X-100 in PBS, blocked in 10% FBS in PBS, immunostained with mouse anti-NP (BioRad: Cat# MCA400) and peroxidase-conjugated rabbit anti-mouse IgG (Santa Cruz; Cat # sc-516102) and visualized with True

(11) Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host DefenseCellApril 30, 2020Zheng M, Karki R, Vogel P, Kanneganti TD.(Santa Cruz Biotechnology, NS1–23-1; sc-130568, 1:1000), anti-lamin A/C (CST; #2032, 1:1000), anti-NP (Bio-Rad, MCA400; 1:1000), anti-β-actin (Proteintech, 66009–1-IG, 1:5000), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories

(12) Influenza Virus Z-RNAs Induce ZBP1-Mediated NecroptosisCellMarch 19, 2020Zhang T, Yin C, Boyd DF, Quarato G, Ingram JP, Shubina M, Ragan KB, Ishizuka T, Crawford JC, Tummers B, et al.PB1,GeneTex,Cat#GTX125923; RRID: AB_2753122 "Mouse monoclonal anti-Influenza A virus NP, Clone AA5H",Bio-Rad,Cat#MCA400; RRID: AB_2151884 Rabbit polyclonal anti-Influenza A virus NP,GeneTex,Cat#GTX125989; RRID: AB_11168364 Rabbit

(13) The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinantNature communicationsDecember 4, 2019Bogdanow B, Wang X, Eichelbaum K, Sadewasser A, Husic I, Paki K, Budt M, Hergeselle M, Vetter B, Hou J, et al.at 4 °C and stained with specific antibody against NP antigen (clone AA5H, Bio-Rad/Serotec, 1:1000, #MCA400). An Alexa Fluor 488-conjugated goat anti-mouse immunoglobulin G (IgG) antibody (Invitrogen) was used

(14) Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B virusesEuropean journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical SciencesMay 15, 2019Zhang J, Hu Y, Hau R, Musharrafieh R, Ma C, Zhou X, Chen Y, Wang J.min. After blocking with 10% bovine serum, cells were stained with mouse anti-NP antibody (Bio-Rad: MCA400) and rabbit anti-M1 (GeneTex: GTX125928) and followed by staining with anti-mouse secondary antibody

(15) RNAi-based small molecule repositioning reveals clinically approved urea-based kinase inhibitors as broadly active antiviralsPLOS PathogensMarch 18, 2019Markus Lesch et al.of the NP protein was investigated by indirect immunofluorescence analysis using primary antibodies MCA400 (IAVs, AbD Serotec), MCA403 (IBVs, AbD Serotec), polyclonal-anti hantavirus N protein and V5507 (VSV,

(16) Unravelling the Role of O-glycans in Influenza A Virus InfectionScientific reportsNovember 6, 2018Mayr J, Lau K, Lai JCC, Gagarinov IA, Shi Y, McAtamney S, Chan RWY, Nicholls J, von Itzstein M, Haselhorst T.background. Primary antibody anti-Influenza A NP mab (final 1 μg/ml; AbD Serotec Influenza A NP mAb, MCA400) in PBS + 1% BSA + 0.05% Tween 20 was then incubated for 1 hr before washing with PBS + 0.05% Tween 20

(17) Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A VirusMBioJuly 24, 2018Kühnl A, Musiol A, Heitzig N, Johnson DE, Ehrhardt C, Grewal T, Gerke V, Ludwig S, Rescher U.Staining with anti-NP antibody (mouse anti-influenza A virus nucleoprotein, clone AA5H; AbD Serotec [MCA400]) for 2 h and anti-mouse Alexa Fluor 594 (donkey anti-mouse IgG [H+L] secondary antibody, Alexa Fluor

(18) NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC UbiquitinationJournal of virologyApril 15, 2018Park HS, Liu G, Thulasi Raman SN, Landreth SL, Liu Q, Zhou Y.goat polyclonal anti-DDDDK tag antibody [ab1257; Abcam], and mouse monoclonal anti-IAV NP antibody [MCA400; Bio-Rad]) overnight at 4°C followed by an incubation with the secondary antibodies (Alexa Fluor 546

(19) Chemical Genomics Approach Leads to the Identification of Hesperadin, an Aurora B Kinase Inhibitor, as a Broad-Spectrum Influenza AntiviralInternational journal of molecular sciencesSeptember 8, 2017Hu Y, Zhang J, Musharrafieh R, Hau R, Ma C, Wang J.After blocking with 10% bovine serum, cells were stained with mouse anti-NP (1:200 dilution; Bio-Rad: MCA400, Hercules, CA, USA) and rabbit anti-M1 (1:200 dilution; Gene Tex: GTX125928) antibodies, followed by

(20) Discovery of dapivirine, a nonnucleoside HIV-1 reverse transcriptase inhibitor, as a broad-spectrum antiviral against both influenza A and B virusesAntiviral researchSeptember 1, 2017Hu Y, Zhang J, Musharrafieh RG, Ma C, Hau R, Wang J.blocking with 10% bovine serum, cells were sequentially stained with mouse anti-NP antibody (Bio-Rad: MCA400), rabbit anti-M1 antibody (Genetex: GTX125928), and anti-mouse secondary antibody conjugated to Alexa-488

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MOUSE ANTI INFLUENZA A NUCLEOPROTEIN

MOUSE ANTI INFLUENZA A NUCLEOPROTEIN from Bio-Rad (Formerly AbD Serotec)

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MOUSE ANTI INFLUENZA A NUCLEOPROTEIN from Bio-Rad (Formerly AbD Serotec)

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