Fig 1: Cell cytotoxicity assay and proliferation assay. (A) CCK-8 assay of cell viability for SK-BR-3 cells incubated with DNS at various concentration. (B) CCK-8 assay of cell viability for SK-BR-3 cells incubated with AptDzy-DNS at different hours. (C) RT-PCR characterization of HER2 mRNA expression for SK-BR-3 cells incubated without and with AptDzy-DNS. The data error bars indicate means ± SD (n = 5). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test), (D) Western blot characterization of HER2 expression for SK-BR-3 cells incubated with 0.25 (Lane 1), 0 (Lane 2), 0.5 (Lane 3), 0.25 (Lane 4), and 0.125 (Lane 5) µM AptDzy-DNS.
Fig 2: Characterization of AptDzy-DNS induced translocation. Confocal microscopy images of SK-BR-3 cells after incubation with 0.5 µM Dzy-DNS without aptamer, HAptDzy-DNS, NAptDzy-DNS, and the mixture of HAptDzy-DNS and NApt-Dzy-DNS for 2 h. Scale bars: 20 µm. (Note: NApt, anti-nucleolin aptamer; HApt, anti-HER2 aptamer; Apt, aptamer).
Supplier Page from Cell Signaling Technology for Phospho-HER2/ErbB2 Antibody Sampler Kit