Fig 1: CBP/ß-catenin promotes the maintenance of stem cell reprogramming by Piwil2. (A) GSEA plot showing significant enrichment of the Wnt/ß-catenin signaling activation modules in HaCaT-Piwil2 cells. (B) Immunoblotting of ß-catenin translocated into the nucleus in HaCaT-Piwil2 cells after treatment with 20 µM ICG-001 or IQ-1 for 24 h. (C) Nuclear lysates from HaCaT-Piwil2 cells treated with 20 µM ICG-001, 20 µM IQ-1, or DMSO were coimmunoprecipitated with antisera to ß-catenin and immunoblotted for CBP and p300. (D and E) The expression of Piwil2 and the “reprogramming” factors c-Myc, Nanog, Oct4, Sox2, and Klf4 was determined by real-time PCR and immunoblotting in HaCaT-Piwil2 cells treated with 20 µM ICG-001, 20 µM IQ-1, or DMSO for 24 h. (F) The proportion of CD49f-, CD338-, OCT4-, and ALDHA1-positive cells, determined by flow cytometry in HaCaT-Piwil2 cells treated with 20 µM ICG-001, 20 µM IQ-1, or DMSO for 24 h. The data are presented as the mean ± SD. *P < 0.05 and **P < 0.01 by Student's t test.