Fig 1: Gating strategy for helper-like ILCs in peripheral blood, adjacent gut, and tumor tissue(A) Gating strategy for human ILCs in peripheral blood. ILCs are defined as CD45+, lineage-negative (Lin-) and CD127-positive (CD127+) singlets. ILC1s are defined as CD45+ Lin- CD127+ CD117- CRTH2-, ILC2s as CD45+ Lin- CD127+ CRTH2+; ILC3s as CD45+ Lin- CD127+ CD117+ CRTH2-. Lineage markers include TCR?d, TCRaß, CD3, CD19, CD14, CD16, CD94, CD123, CD34, CD303 and FceRI.(B) Gating strategy of human ILCs in adjacent gut.(C) Gating strategy of human ILCs in tumor tissue. SSC, side scatter; FSC: forward scatter. PBMC, peripheral blood mononuclear cells. Arrows indicating that the gating is always from the drawn gate on the subsequent plot.