Fig 1: Effect of intra-lymphatic injection of GAD-alum (n = 12) on T cells and cells with regulatory phenotype. (A) Median percentage of activated (CD25+CD127+) CD4+ (blue triangles) and CD8+ (orange circles) T cells after stimulation with GAD65 (5 µg/ml). (B) Gating strategy for naïve (TN, CD45RA+CCR7+, gray circles), central memory (TCM, CD45RA-CCR7+, orange squares), and effector memory (TEM, CD45RA-CCR7- and CD45RA+CCR7-, blue triangles) cells, and median percentage within CD4+ (up) and CD8+ (down) T cells. (C) Representative dot plot showing the expression of FOXP3 and CD25 on CD4+ T cells and median percentage of regulatory T cells within CD4+ T cells. (D) Gating strategy and median percentages for non-suppressive (nsTreg, CD45RA-FOXP3low, blue circles), resting (rTreg, CD45RA+FOXP3low, gray squares), and activated (aTreg, CD45RA-FOXP3high, orange triangles) regulatory T cells. Bars indicate interquartile range. Differences between time-points within the same group were calculated using Wilcoxon paired test.