Fig 1: Stem cell features, markers and immunolocalization of a capillary resident regenerative population (CRP).a Expression of selected stem or progenitor cell-related genes. b Signaling entropy rate (entropy) for each BEC subset. Highest 1% of all BEC, dashed box. High entropy cells are enriched in early CRP. c tSpace projection from Supplementary Fig. 1d with all cells. CRP, green. High Entropy cells (top 1% as gated in (b)) are black. Other cells, gray. Interactive rendering available: https://stanford.io/2WXR811 d Percent of each BEC subset classified as dividing based on pooled expression of cell-cycle genes. Points represent values for individual samples. Diamonds, average of all samples. Error bars, standard error of the mean. n = 3 biologically independent samples. e Phenotype of dividing cells presented as percent of dividing cells with the indicated BEC phenotypes. Error bars, standard error of the mean. n = 3 biologically independent samples. f Experimental timeline for (g) and (h). g Quantification of ER+ endothelium by immunofluorescence histology in resting (day 0) PLN and in PLN 5 days after cutaneous inflammation. Expressed as ER+ capillaries (PODXL+ or MCAM+ PNAd-EC) or ER+ HEV (PNAd+) as percent of capillary EC or HEC units (arbitrary units of length) scanned. No ER+ HEC were detected out of over 5000 scanned in resting and over 5000 in inflamed PLN. 95% CL are shown as error bars or indicated. For each group, nine lymph nodes from two mice were enumerated. Each datum is from a given LN. Statistically significant differences were determined using a Kruskal-Wallis test and Dunn’s multiple comparisons test. 95% confidence limits are shown as error bars or indicated above HEC. h Representative images of resting PLN from AplnCreER mice stained with anti-PNAd (yellow), anti-PODXL (red), anti-ER (green) and intravenously injected anti-MCAM (blue). Arrow heads point to ER+ CRP. Bars, 50 µm. Images representative of three independent experiments. i Images of resting PLN (72 h post 4-OHT) from AplnCreER, mTmG mice stained with injected anti-CXCR4 (blue), anti-CD276 (white) and anti-EMCN (red). Arrow heads point to GFP+ cells in capillaries. Bars, 20 µm. Images representative of three independent experiments.