Fig 1: Validation of the efficacy of the aminooxy-biotin based glycocapture method. (A) Western blot experiment performed on total cell lysates from human CPC and MSC cultures. After SDS–PAGE, protein bands were visualised using the Odyssey FC imaging system (Li-Cor) and bands were detected using the 800 nm (for KCNMA1) and 700 nm (for ß-actin) channels. Optical density values of detected bands were calculated using the Image Studio software and the values for KCNMA1 were normalised to those of ß-actin and then to MSC. Representative gel image. Full-length uncropped blots are presented in Fig. S2. Error bars are ± SD, n = 3. * indicates statistical significance (P < 0.05). (B) Intracellular distribution of KCNMA1 in MSCs and CPCs detected by immunocytochemistry. Primary antibodies were visualised using Alexa488-conjugated anti-rabbit secondary antibodies. Nuclear DNA was stained with DAPI. Data shown are representative out of 3 independent experiments. Scale bar, 50 µm.