Fig 1: Transduction efficiency of CPEGAd in the presence of NAbs and CAR-independent cellular uptake. A Flow cytometry quantification of PANC-1 GFP-positive cells after infection with naked or CPEG-coated GFP-expressing reporter AdGFPLuc at MOI 50 in the presence or absence of nAbs. The positive symbol (+) represents pre-incubation of virus samples with 1 × 104 dilution of commercial anti-Ad5 antibody (ab6982, Abcam). B Representative fluorescent images of A549 and MCF-7 cells transduced at MOI 100 of naked Ad or CPEGAd. The percentage of GFP-positive cells resulting from flow cytometry assay is indicated. C Infectivity of CPEG-coated AdGFPLuc in CAR+ (A549) and CAR- (MCF-7) cells. Five thousand cells/well were infected with naked Ad and CPEGAd at increasing MOIs ranging from 0 to 4500, and the percentage of GFP-positive cells was analyzed by flow cytometry. The MOI needed to achieve 15% of GFP-positive cells was determined and is shown for each condition. *P < 0.05, ** P < 0.01, *** P < 0.001
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