Fig 2: The fluorescent targets and their corresponding antibody dilutions are as follows; for AT1—extracellular-ATTO Fluor-550; AT2—extracellular-ATTO Fluor-488 and MAS receptor –Fluor-488 tags (1° antibody delusion 1:50) were visualized by confocal microscopy. The 4', 6-diamidino-2-phenylindole and F-actin merged images show the green fluorescence is specific for angiotensin receptors. Images were adjusted for brightness/contrast using the Zeiss LSM 800 image browser similar for all 3 panels. Fluorescence intensity was quantified by ImageJ software and cumulative data are presented in a bar graph. Values in each panel are mean ± SEM; *P < 0.05.

Fig 3: Expression of mRNAs was studied by RT–PCR. Relative gene expression of AT1, AT2, MAS, and NOX4 mRNA profile in the SCG normalized to ß-actin expression by the 2??CT comparative method and expressed as fold change compared with HnSD. Values are mean ± SEM. mRNA for AT2 is significantly lower in SCG of (mRen2)27 transgenic hypertensive animals compared with control (1.013 ± 0.105 vs. 0.171 ± 0.013 a.u.; n = 6; *P < 0.05).

Fig 4: Protein expression of AT1, AT2 receptor subtypes, Ang-(1-7)-mediated MAS receptor, and NOX4-specific protein density in SCG. AngII receptor density was measured by western blot hybridization using specific antibodies in ganglia isolated from (mRen2)27 hypertensive and HnSD rats. Top: Densitometry analyses of protein level normalized to ß-actin. Image analyses of the signals are normalized to ß-actin. Values in each panel are mean ± SEM; *P < 0.05. Representative western blot. Bottom: Representative western blot, respectively.