Fig 1: Expression of UBR5–ZNF423 fusion transcripts and protein in C666-1. Exon-specific gene expression analysis of whole-transcriptome sequencing (a) and quantitative RT–PCR (b) revealed the over-expression of exons 7–9 of ZNF423 in C666-1. NP69 (immortalized normal nasopharyngeal epithelial cells) and HK1 (EBV-negative well differentiated NPC cell line) were recruited as additional references. 5'-Z (Hs01046870_m1, Applied Biosystems), M-Z (Hs00391820_m1, Applied Biosystems), and 3'-Z (Hs00323880_m1, Applied Biosystems) indicate the regions in ZNF423 assessed by quantitative RT–PCR assay. (c) Predicted amino acid sequences and domain of UBR5–ZNF423 fusion protein: for the predicted amino acid sequences, the amino acids derived from UBR5 sequences are highlighted in blue; the zinc finger sequences are underlined. (d) A chimeric UBR5–ZNF423 protein of approximately 10.8 kDa (red arrow) was detected in C666-1 cells by western blotting. Full-length ZNF423 protein is not expressed in either C666-1 or the immortalized nasopharyngeal epithelial cells NP69. HT1080 and HeLa are positive and negative controls, respectively, for wild-type ZNF423 expression. The amino acid sequences of the UBR5–ZNF423 fusion protein specific for the anti-ZNF423 antibody (ab4451; Abcam) is highlighted in yellow.