Fig 2: Assays of cell proliferation, caspase 3 activity, PCNA and MMP9 expression after 48h transfection with PC-1 siRNA.A: Under the microscope; B: BrdU Cell Proliferation assay; C: Caspase 3 activity assay; D: PCNA mRNA expression (qRT-PCR); E: MMP9 mRNA expression (qRT-PCR); A&B: Compared with the control siRNA group, the number of A2780 and SKOV3 cells transfected with PC-1 siRNA was significantly reduced, and the cell proliferation rate decreased significantly; C: Caspase 3 activity of A2780 and SKOV3 cells transfected with PC-1 siRNA was increased 2.79 and 2.07 fold; D: the expression of PCNA mRNA in A2780 and SKOV3 was down regulated by 55.47% and 39.19%; E: the expression of MMP9 mRNA in A2780 and SKOV3 was down regulated by 62.97% and 46.98%. *P<0.05.

Fig 3: Detection of migration and invasion of A2780 cells after 48h transfection with PC-1 siRNA.A: wound-healing assay; B: Transwell migration assay; C: Matrigel invasion assay; D: quantification for transwell migration assay; E: quantification for matrigel invasion assay; F: E-cadherin mRNA expression (qRT-PCR); G: N-cadherin mRNA expression (qRT-PCR); H: vimentin mRNA expression (qRT-PCR); *P<0.05.

Fig 4: ENPP1 mRNA and protein expression of A2780 after 48h of PC-1 siRNA transfection.A: Western Blot electrophoresis; B: Analysis of protein relative expression. C: qRT-PCR. Blank: A2780 cells was interfered by cell culture medium without any siRNA; Control siRNA: A2780 cells was interfered by Control siRNA; PC-1 siRNA: A2780 cells was interfered by PC-1 siRNA. *P<0.05.