Anti-Neutrophil antibody [NIMP-R14] ab2557 from Abcam

Product Specs
ItemAnti-Neutrophil antibody [NIMP-R14]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2557
Antibody TypePrimary
Quantity50 UG
IsotypeIgG2b
ApplicationsFlow Cytometry, Functional Studies, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections)
HostRat
CloneNIMP-R14
ClonalityMonoclonal
Conjugate/TagUnconjugated
Concentration0.1 mg/ml
Gene NameLy6b
ReactivityMouse
TargetNeutrophil

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The RNA binding proteins ZFP36L1 and ZFP36L2 are dysregulated in airway epithelium in human and a murine model of asthmaFrontiers in Cell and Developmental BiologyOctober 19, 2023Jennifer Rynne, Elena Ortiz-Zapater, Dustin C. Bagley, Onofrio Zanin, George Doherty, Varsha Kanabar, Jon Ward, David J. Jackson, Maddy Parsons, Jody Rosenblatt, et al.blocking solution for all primary antibodies used: mouse anti-Muc5ac (Abcam ab3649), rat anti-Ly-6G (Abcam ab2557), rabbit anti-Zfp36l1/l2 (Cell Signaling Technologies). Ex vivo lung slices were washed 3 × 30 min in

(2) Macrophage CARD9 mediates cardiac injury following myocardial infarction through regulation of lipocalin 2 expressionSignal Transduction and Targeted TherapyOctober 13, 2023Yan Liu, Yi-hui Shao, Jun-meng Zhang, Ying Wang, Mei Zhou, Hui-qin Li, Cong-cong Zhang, Pei-jie Yu, Shi-juan Gao, Xue-rui Wang, et al.Santa Cruz Biotechnology; anti-CD31 (DIA-310) from Dianova; anti-MMP9 (ab38898 and ab283575), neutrophils (ab2557), F4/80 (ab6640), and SLC22A17 (ab124506) from Abcam; anti-α-actinin (A7811) from Sigma-Aldrich; anti-LCN2

(3) Humulus japonicus ameliorates irritant contact dermatitis by suppressing NF‑κB p65‑dependent inflammatory responses in miceExperimental and Therapeutic MedicineAugust 2, 2023Yu-Bin Kim, Eun-Jung Kang, Jung-Ran Noh, Jin-Pyo An, Jong-Tae Park, Won Keun Oh, Yong-Hoon Kim, Chul-Ho LeeLaboratories, Inc.) for 30 min at RT, and then stained with NIMP-R14 anti-neutrophil antibody (1:200; cat. no. ab2557; Abcam) and anti-F4/80 antibody (1:500; cat. no. 70076S; Cell Signaling Technology, Inc.) overnight

(4) Interferon-γ elicits the ocular surface pathology mimicking dry eye through direct modulation of resident corneal cellsCell Death DiscoveryJune 30, 2023Jung Hwa Ko, Seonghwan Kim, Jin Suk Ryu, Hyo Jeong Song, Joo Youn Ohimmunofluorescence staining, rat monoclonal anti-mouse neutrophil antibody for murine Gr-1 (Cat No. ab2557, Abcam) was used as a primary antibody, and goat polyclonal anti-rat IgG antibody (Cat No, A10522, Invitrogen)

(5) T follicular helper cells contribute to pathophysiology in a model of neuromyelitis optica spectrum disordersJCI InsightFebruary 22, 2023Leung-Wah Yick, Oscar Ka-Fai Ma, Ethel Yin-Ying Chan, Krystal Xiwing Yau, Jason Shing-Cheong Kwan, Koon-Ho Chancatalog AB55811, Abcam), rat anti–lymphocyte antigen 6 complex locus G6D (anti-Ly6G; 1:400; catalog AB2557, Abcam), rat anti–Siglec-F (1:50; catalog 552125, BD Biosciences), mouse anti–cluster of differentiation

(6) TLR4 Blockade Using Docosahexaenoic Acid Restores Vulnerability of Drug-Tolerant Tumor Cells and Prevents Breast Cancer Metastasis and Postsurgical RelapseACS Bio & Med Chem AuFebruary 15, 2023Mou Wang, Yuejing Wang, Renhe Liu, Ruilian Yu, Tao Gong, Zhirong Zhang, Yao FuP01375), Ki67 (Servicebio, GB13030-2) (UniProtKB E9PVX6), F4/80 (Abcam, ab100790), neutrophil (Abcam, ab2557) (UniProtKB Q9Z1Q4), and CD34 (Abcam, ab187282) (UniProtKB P28906) at 4 °C overnight, followed by biotinylated

(7) Inflammation Confers Healing Advantage to Corneal Epithelium Following Subsequent InjuryInternational Journal of Molecular SciencesFebruary 7, 2023Jin Suk Ryu, So Yeon Kim, Mee Kum Kim, Joo Youn OhMA, USA) or neutrophil immunostaining. For neutrophil staining, a rat anti-mouse neutrophil antibody (ab2557; Abcam, Cambridge, MA, USA) and a goat anti-rat IgG TRITC (AP136R, Sigma-Aldrich) were used as primary

(8) Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic CancerCancer immunology researchJanuary 3, 2023Faraoni EY, O'Brien BJ, Strickland LN, Osborn BK, Mota V, Chaney J, Atkins CL, Cen P, Rowe J, Cardenas J, et al.(Cell Signaling Technology, catalog no. 9664), Granzyme B (Abcam, catalog no. ab255598), NIMPR14 (Abcam, catalog no. ab2557), myeloperoxidase (MPO; Abcam, catalog no. ab208670), αSMA (Abcam, catalog no. ab5694), CD31 (Abcam,

(9) Prolonged oral antimicrobial administration prevents doxorubicin-induced loss of active intestinal stem cellsGut MicrobesDecember 31, 2022Breanna J Sheahan, Casey M Theriot, Jocsa E. Cortes, Christopher M Dekaneyantibody, rat anti-F4/80 (1:200, ab6640, Abcam, Cambridge, MA, USA) or rat anti-neutrophil (1:250, ab2557, Abcam, Cambridge, MA, USA), washed in 1x PBS, and incubated for 1 hour at room temperature with secondary

(10) Comparison of the pathogenesis of SARS-CoV-2 infection in K18-hACE2 mouse and Syrian golden hamster modelsDisease models & mechanismsNovember 1, 2022Jeong H, Woo Lee Y, Park IH, Noh H, Kim SH, Kim J, Jeon D, Jang HJ, Oh J, On D, et al.Thermo Fisher Scientific, 1:1000), anti-PTPRC (ab64100, Abcam, 1:1000), anti-CD3D (ab5690, Abcam, 1:200), anti-Ly-6G/Ly-6C (ab2557, Abcam, 1:250), anti-p-AKT (9271S, Cell Signaling Technology, 1:500), anti-CHGA (ab15160, Abcam, 1:1000),

(11) A neutrophil-B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4eLifeSeptember 30, 2022Gawish R, Maier B, Obermayer G, Watzenboeck ML, Gorki AD, Quattrone F, Farhat A, Lakovits K, Hladik A, Korosec A, et al.monoclonal),BioLegend,Cat# 146507; RRID:AB_2562784,FC ‘(1:200)’ Antibody,anti-mouse NIMP-R14 (rat monoclonal),Abcam,Cat# ab2557-50; RRID:AB_303154,IHC ‘(1:50)’ Antibody,Biotin anti-rat IgG (goat polyclonal),BioRad,Cat# STAR131B;

(12) ZnT2 Is Critical for TLR4-Mediated Cytokine Expression in Colonocytes and Modulates Mucosal Inflammation in MiceInternational journal of molecular sciencesSeptember 28, 2022McGourty K, Vijayakumar R, Wu T, Gagnon A, Kelleher SL.Abcam, Burlingame, CA, USA, ab13556), anti-neutrophil antibody (1:100; Abcam, Burlingame, CA, USA, ab2557), and anti-8OHdG (5 µg/mL; Abcam, Burlingame, CA, USA, ab48508). Secondary antibodies used were Alexa

(13) Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine TissuesMethods and ProtocolsSeptember 19, 2022Stephanie Arnold, Sarah Watts, Emrys Kirkman, Clive P. Page, Simon C. PitchfordFFPE,EDTApH 8 MPO,"Abcam, UK",ab90810,mouse,"1/50, 1/100 1/500",×,N/A Neutrophil (NIMP-R14),"Abcam, UK",ab2557,rat,1/100 1/500,×,N/A PSGL-1,"Abcam, UK",ab110096,rat,"1/50, 1/500",×,N/A Neutrophil-specific antibodies

(14) Chronic suppurative otitis media causes macrophage-associated sensorineural hearing lossJournal of neuroinflammationSeptember 12, 2022Xia A, Thai A, Cao Z, Chen X, Chen J, Bacacao B, Bekale LA, Schiel V, Bollyky PL, Maria PLS.MN, USA), rat anti-F4/80 (1:150, ab6640, Abcam Inc., Cambridge, MA, USA), rat anti-Ly-6G/C (1:100: ab2557, Abcam Inc, USA). The specimens were incubated with secondary antibodies diluted in 0.1% Triton-X 100,

(15) Restoring Prohealing/Remodeling-Associated M2a/c Macrophages Using ON101 Accelerates Diabetic Wound HealingJID InnovSeptember 1, 2022Lin CW, Chen CC, Huang WY, Chen YY, Chen ST, Chou HW, Hung CM, Chen WJ, Lu CS, Nian SX, et al.(E-AB-70051, Elabscience, Houston, TX), CD206 (ab64693, Abcam), cytokeratin 14 (ab181595, Abcam), Ly6G/6C (ab2557, Abcam), MMP9 (ab228402, Abcam), CD3 (ab16669, Abcam), MOMA2 (ab33451, Abcam), and αSMA (ab32575, Abcam)." mouse,Flow,"APC-conjugated

(16) TRIM18 is a critical regulator of viral myocarditis and organ inflammationJournal of biomedical scienceJuly 31, 2022Fang M, Zhang A, Du Y, Lu W, Wang J, Minze LJ, Cox TC, Li XC, Xing J, Zhang Z.subsequently incubated overnight at 4 °C with the primary antibodies including anti-neutrophil Rat antibody (ab2557, Abcam), anti-macrophage Rat antibody (ab56297, Abcam), anti-NK cell marker mouse antibody (sc-59340,

(17) Maternal and perinatal obesity induce bronchial obstruction and pulmonary hypertension via IL-6-FoxO1-axis in later lifeNature communicationsJuly 27, 2022Selle J, Dinger K, Jentgen V, Zanetti D, Will J, Georgomanolis T, Vohlen C, Wilke R, Kojonazarov B, Klymenko O, et al.anti-CD45 (BD, #550539, Clone 30-F11, 1:25, Franklin Lakes, USA) or monoclonal rat anti-LyG6 (Abcam, #ab2557, 1:50, Cambridge, UK) was applied for 30 min. For LyG6 immunostaining, tissues were pre-treated with

(18) Fat and exposure to 4-nitroquinoline-1-oxide causes histologic and inflammatory changes in murine liversPLOS ONEMay 31, 2022Lenore D. Pitstick, Joanna Goral, Ryan A. Schmelter, Christine M. Fuja, Mae J. Ciancio, Matthew Pytynia, Alice Meyer, Jacalyn M. GreenCambridge, MA); rabbit anti-CD68 (1:2000, ab125212, lot number GR77836-39, Abcam); or rat anti-Ly6 (1:500, ab2557, lot number GR277603-1, Abcam). Primary antibodies were diluted in 2.5% horse or goat serum/TBS. Specificity

(19) Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanismNature medicineApril 1, 2022Monteiro C, Miarka L, Perea-García M, Priego N, García-Gómez P, Álvaro-Espinosa L, de Pablos-Aragoneses A, Yebra N, Retana D, Baena P, et al.(1:500; ab732, Abcam), S100A8 (1:200; ab92331, Abcam), Iba1 (1:500; 19-19741, Wako), NIMP-R14 (1:100; ab2557, Abcam), TGF-α (1:100; ab9585, Abcam), Topo IIα (1:100; sc-365916, Santa Cruz Biotechnology), transglutaminase

(20) Dissecting the Balance Between Metabolic and Oncogenic Functions of Astrocyte-Elevated Gene-1/MetadherinHepatology communicationsMarch 1, 2022Rajesh Y, Reghupaty SC, Mendoza RG, Manna D, Banerjee I, Subler MA, Weldon K, Lai Z, Giashuddin S, Fisher PB, et al.#NB110‐40877; 1:200); F4/80 antibody (rat; Bio‐Rad #MCA497R; 1:100), and anti‐neutrophil antibody (rat; Abcam #ab2557; 1:200). Hepatocytes were cultured in collagen‐1‐coated four‐chamber slides, and immunofluorescence (IF

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Anti-Neutrophil antibody [NIMP-R14] from Abcam

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Anti-Neutrophil antibody [NIMP-R14] from Abcam

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