Fig 2: Elevated Dystroglycan Correlates with GBM Patient Outcome and aDG is Abundantly Glycosylated in GBM. aDAG1 expression was correlated with GBM patient survival using the Rembrandt (n = 523) and TCGA (n = 453) databases. b QPCR analysis of DAG1, ITGA6, EPHA2 and EPHA3 mRNA expression in GBM tissue specimens (n = 28). c Flow cytometric analysis for aDG glycosylation (IIH6 mAb) was performed on primary GBM cell lines grown as serum-free GNS cultures, compared to isotype control. See also Online Research 1d for full analysis. d aDG glycosylation was assessed by western blot in four primary GBM cell lines. e aDG glycosylation was assessed by western blot following cell fractionation to compare cytoplasmic versus membrane localisation. ß-actin was used as a loading control. f Flow cytometric analysis was performed for aDG glycosylation (IIH6 mAb) and EphA3 (IIIA4 mAb) in 10 early passage primary GBM cultures, mean channel fluorescence (mcf) was used to determine the correlation coefficient between EphA3 and glycosylated aDG (r = 0.899). GBM subtypes: MES mesenchymal, PN proneural, CL classical