Fig 2: mTOR and P70S6K expression levels in muscle tissues from the different groups. (A) Immunohistochemistry analysis of mTOR (arrows show mTOR-immunoreactive cells) and (B) p70S6K protein expression (arrows show p70S6K-immunoreactive cells). Scale bar, 50 µm. (C) Western blot analysis of mTOR and (D) p70S6K protein expression levels. GAPDH was used as an internal control. Data are presented as the mean ± standard error of the mean. *P<0.05 vs. normal group; #P<0.05 vs. ES group (n=10 per group). mTOR, mammalian target of rapamycin; p70S6K, p70 ribosomal S6 protein kinase; ES, electrical stimulation.