AMV Reverse Transcriptase from Promega

Product Specs
ItemAMV Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM5101
Quantity300u
Concentration10 U/µl

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml BSA, 1mM radiolabeled dTTP and 0.25mM poly(A)400 and 0.25mM oligo (dT)50.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Identification of bioactive compounds of Bacillus velezensis HNA3 that contribute to its dual effects as plant growth promoter and biocontrol against post-harvested fungiMicrobiology SpectrumDecember 12, 2023Doaa S. Zaid, Wenya Li, Siyu Yang, Youguo LiFirst strand cDNA synthesis has been done for RNA samples using the Promega cDNA Synthesis AMV Reverse Transcriptase Kit cat# M5108 according to the manufacturer’s instructions. cDNA synthesis was performed on Bio-Rad100 Thermal cycler

(2) A flow cytometry-based assay to investigate HIV-1 expression in SMYD5 shRNA containing primary CD4+ T cellsSTAR ProtocolsDecember 1, 2023Daniela Boehm, Melanie OttT-activator CD3/CD28,Life Technologies,Cat# 11131D; Lot# 00979236 AMV reverse transcriptase,Promega,Cat# M5101 Maxima SYBR Green PCR master mix,Thermo Scientific,Cat# K0253 X-tremeGENE 9 DNA transfection reagent,Roche,Cat#

(3) New proapoptotic chemotherapeutic agents based on the quinolone-3-carboxamide scaffold acting by VEGFR-2 inhibitionScientific ReportsJuly 13, 2023Zeinab S. El-Fakharany, Yassin M. Nissan, Nada K. Sedky, Reem K. Arafa, Sahar M. Abou-SericDNA synthesis was performed for RNA using the Promega cDNA Synthesis AMV Reverse Transcriptase Kit (cat# M5108) according to the manufacturer’s instructions. The reaction components were added as shown in Table

(4) HPr prevents FruR-mediated facilitation of RNA polymerase binding to the fru promoter in Vibrio choleraeNucleic Acids ResearchJune 23, 2023Chang-Kyu Yoon, Seung-Hwan Lee, Jing Zhang, Hye-Young Lee, Min-Kyu Kim, Yeong-Jae SeokTable S2), and then extended using 5 units of AMV reverse transcriptase (Promega, Madison, WI, USA, #M5101) for 1 h at 42°C following the manufacturer’s instructions. A 133-bp HEX-labelled DNA prepared with

(5) The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11Cell reportsMarch 28, 2023Boehm D, Lam V, Schnolzer M, Ott M.Recombinant",Thermo Fisher Scientific,Cat# PV4131; Lot# 1860128B AMV Reverse Transcriptase,Promega,Cat# M5101 SYBR Green PCR Master Mix,Applied Biosystems,Cat# 4309155 X-tremeGENE 9 DNA Transfection Reagent,Roche,Cat#

(6) Post‐prostatic‐massage urine exosomes of men with chronic prostatitis/chronic pelvic pain syndrome carry prostate‐cancer‐typical micro RNA s and activate proto‐oncogenesMolecular OncologyMarch 1, 2023Laura Schneider, Temuujin Dansranjav, Elena Neumann, Hang Yan, Adrian Pilatz, Hans‐Christian Schuppe, Florian Wagenlehner, Undraga Schagdarsurenginreverse‐transcribed into cDNA using AMV reverse transcriptase (Promega, Fitchburg, WI, USA; cat. no M5101) and random‐hexamer primers, and amplified using PowerUp™ SYBR® Green Mastermix (Thermo Fisher Scientific;

(7) Nonstructural protein 1 widespread RNA decay phenotype varies among coronavirusesiScienceJanuary 20, 2023Bermudez Y, Miles J, Muller M.TRIzol Reagent,ThermoFisher,15596026 Turbo DNase,Invitrogen,AM2238 AMV Reverse Transcriptase,Promega,PR-M5108 Q5® High-Fidelity DNA Polymerase,New England Biolabs,M0491S Doxycycline,BD Biosciences,NC0424034 Polybrene,FISHER,Cat#

(8) DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion productionNucleic acids researchJanuary 11, 2023Hartenian E, Mendez AS, Didychuk AL, Khosla S, Glaunsinger BA.,New England Biolabs,R0138, PstI-HF,New England Biolabs,R3140, Turbo Dnase,ThermoFisher,AM2238, AMV RT,Promega,M5101, iTaq SYBR MasterMix,BioRad,1725125, SYBR Safe,Invitrogen,S33102, Proteinase K,promega,MC5005, Rnase A

(9) Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and deathCell reportsOctober 18, 2022Aber ER, Griffey CJ, Davies T, Li AM, Yang YJ, Croce KR, Goldman JE, Grutzendler J, Canman JC, Yamamoto A.assays,Critical commercial assays,Critical commercial assays ,, AMV Reverse Transcription System,Promega,M5101 Papain dissociation system,Worthington,PDS mCLING labeling kit,Synaptic Systems,710-MCK DC protein assay

(10) N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein productsCell ReportsAugust 30, 2022Kyusik Q. Kim et al.Lysis Buffer,Promega,Cat#E1941 M-MLV Reverse Transcriptase,Promega,Cat#M1701 AMV Reverse Transcriptase,Promega,Cat#M5101 EasyTag™ L-[35S]-Methionine,PerkinElmer,Cat#NEG709A500UC "ATP, [γ-32P]",PerkinElmer,Cat#NEG002A100UC 3′-O-Me-m7G(5’)ppp(5′)G RNA Cap Structure Analog

(11) Multi-step recognition of potential 5' splice sites by the Saccharomyces cerevisiae U1 snRNPeLifeAugust 12, 2022Sarah R Hansen, David S White, Mark Scalf, Ivan R CorrÃªa, Lloyd M Smith, Aaron A Hoskins,New England BioLabs,Cat. No. S1404S, "Chemical compound, drug",AMV Reverse Transcriptase,Promega,Cat. No. M5101, "Chemical compound, drug",RnaseH (2 U/μL),Thermo Fisher Scientific,Cat. No. 18021014, "Chemical compound,

(12) Initiator tRNA lacking 1-methyladenosine is targeted by the rapid tRNA decay path way in evolutionarily distant yeast speciesPLOS GeneticsJuly 28, 2022Monika Tasak, Eric M. Phizickydescribed [76], in 10 μL reactions containing 2 U AMV reverse transcriptase (Promega, Madison, WI cat# M5101), 1X AMV RT buffer, 1 mM ddNTP, and 1 mM of the other three dNTPs. Following extension for 1 h at 50°C,

(13) MicroRNA-27b-3p down-regulates FGF1 and aggravates pathological cardiac remodellingCardiovascular ResearchJuly 20, 2022Guoqi Li, Yihui Shao, Hong Chang Guo, Ying Zhi, Bokang Qiao, Ke Ma, Yong-Qiang Lai, Yong Qiang Lai, Yulin Lifirst-strand cDNA synthesis with avian myeloblastosis virus reverse transcriptase (Promega, Catalogue: M5108, Madison, USA), according to manufacturer’s protocol. Special Taqman probes were used for cDNA synthesis

(14) Substituted Syndecan-2-Derived Mimetic Peptides Show Improved Antitumor Activity over the Parent Syndecan-2-Derived PeptideInternational Journal of Molecular SciencesMay 24, 2022Bohee Jang et al.DEPC-treated water. Approximately 3 μg of RNA was used to generate cDNA using AMV Reverse Transcriptase (Cat# M5108) and Random Primer (Cat# C1181) (both from Promega US, Madison, WI, USA). Aliquots of the resulting

(15) The interplay between SARS-CoV-2 infected airway epithelium and immune cells modulates regulatory/inflammatory signalsiScienceFebruary 18, 2022Bordoni V, Matusali G, Mariotti D, Antonioli M, Cimini E, Sacchi A, Tartaglia E, Casetti R, Grassi G, Notari S, et al."Bio-Plex Pro Human Inflammation Panel 1, 37-Plex",Bio-Rad,Cat.#171AL001M Direct-zol RNA MicroPrep KIT,Zymo Research,Cat.#R1051 AMV-reverse transcriptase,Promega,Cat.#M5108 Qiamp viral RNA kit,Qiagen,Cat.#52904 RealStar SARS-CoV-2 RT-PCR Kit,Altona Diagnostics,Cat.#821005 FastStart

(16) Pseudouridine synthases modify human pre-mRNA co-transcriptionally and affect pre-mRNA processingMolecular cellFebruary 3, 2022Martinez NM, Su A, Burns MC, Nussbacher JK, Schaening C, Sathe S, Yeo GW, Gilbert WV.2000,Invitrogen,11668027 Benzonase Nuclease,Millipore Sigma,E1014-25KU T4 PNK,NEB,M0201L T4 DNA Ligase,NEB,M0202L AMV RT,Promega,M5108 MMLV RT,NEB,M0253S Taq Polymerase,NEB,M0273S Phusion Polymerase,NEB,M0530L Doxycycline hyclate,Sigma-Aldrich,D9891-1G Puromycin dihydrochloride

(17) Quantifying full-length circular RNAs in cancerGenome ResearchDecember 1, 2021Ken Hung-On Yu et al.kit (Qiagen 217004). First-strand cDNA was generated with M-MLV (Invitrogen 18080044) or AMV (Promega M5101) reverse transcriptase. The cDNA products were then amplified by PCR with a KAPA2G fast HotStart PCR kit

(18) Triamcinolone acetonide modulates TGF‑β2‑induced angiogenic and tissue‑remodeling effects in cultured human retinal pigment epithelial cellsMolecular medicine reportsNovember 1, 2021Hsiao CC, Chang YC, Hsiao YT, Chen PH, Hsieh MC, Wu WC, Kao YH.Scientific, Inc.) and cDNA was generated using avian myeloblastosis virus reverse transcriptase (cat. no. M5108; Promega Corporation) and oligo-dT primers (cat. no. C1101; Promega Corporation) in the presence of

(19) The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expressionCell reportsOctober 19, 2021Mendez AS, Ly M, González-Sánchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA.mix),Clontech (TaKaRa),638943 TURBO DNase,Invitrogen,"AM2239 (5,000U)" AMV Reverse Transcriptase,Promega,M5101 (300U) SuperScript IV RT,ThermoFisher,"18090050 (10,000U)" PreScission protease,Cytiva,27084301 T7 RNA polymerase

(20) Pseudouridine site assignment by high-throughput in vitro RNA pseudouridylation and sequencingMethods in EnzymologySeptember 14, 2021Nicole M. Martinez, Cassandra Schaening-Burgos, Wendy V. GilbertS-11494) 10x RT buffer without Mg2+ (see recipe) 10 mM dNTPs 100 mM MgCl2 AMV RT (Promega, cat. no. M5108) 1 N NaOH 1 N HCl CircLigase ssDNA ligase (Epicentre, cat. no. CL4115K) 1 mM ATP 50 mM MnCl2 Phusion

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