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Amino Acid Mixtures
Amino Acid Mixture, Complete
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Amino Acid Mixture, Complete
Amino Acid Mixture, Complete
Amino Acid Mixture, Complete from Promega
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Citations:
(19)
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Amino Acid Mixture, Complete
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Description
Amino Acid Mixture, Complete, is an aqueous solution containing 1mM each of the 20 essential amino acids
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Amino Acid Mixture, Complete
Company
Promega
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
L4461
Quantity
175microl
Molecular Weight
~74kDa
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (19)
(1)
Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms
Cell Death & Disease
July 26, 2023
Laura Ryder, Frederic Schrøder Arendrup, José Francisco Martínez, Goda Snieckute, Chiara Pecorari, Riyaz Ahmad Shah, Anders H. Lund, Melanie Blasius, Simon Bekker-Jensen
(Sigma Aldrich, #10127566001), 10 µM spermidine (Sigma Aldrich, # S0266), 10 µM amino acids (
Promega
, #
L4461
), 65 mM KAc, 0.75 mM MgAc2 and murine RNase inhibitor A dual mRNA construct with a Renilla luciferase
(2)
Human-rabbit Hybrid Translation System to Explore the Function of Modified Ribosomes
BIO-PROTOCOL
July 5, 2023
Eriko Matsuura-Suzuki, Hirotaka Toh, Shintaro Iwasaki
catalog number: 16229-95, stored at room temperature)Amino acid mixtures (
Promega
, catalog number:
L4461
, stored at -80 °C)Recombinant RNase inhibitor (TaKaRa, catalog number: 2313A, stored at -20 °C)
Dual-Luciferase Reporter Assay System
(3)
Production of human translation-competent lysates using dual centrifugation
RNA Biology
December 31, 2022
Lukas-Adrian Gurzeler, Jana Ziegelmüller, Oliver Mühlemann, Evangelos D. Karousis
0.68 mM MgCl2, 54.05 mM KCl, 0.05 mM amino acid mixture, unless otherwise stated (
Promega
, Cat. No.
L4461
), 13.51 mM Creatine Phosphate, 229.73 ng/ml Creatine kinase and 1x protease
inhibitor
cocktail (Bimake,
(4)
Functional characterization of 5′ UTR cis-acting sequence elements that modulate translational efficiency in Plasmodium falciparum and humans
Malaria Journal
December 1, 2022
Valentina E. Garcia, Rebekah Dial, Joseph L. DeRisi
384-well plates. A master mix of 3.5 µL lysate with 0.5 µL 100 μm complete amino acid mix (
Promega
L4461
) and 1uL 10x translation buffer (20 mM HEPES pH 8, 75 mM KoAc, 2 mM DTT, 5 mM ATP, 1 mM GTP 200 mM creatine
(5)
PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunity
Cell
August 18, 2022
Wang J, Zhang X, Greene GH, Xu G, Dong X.
#TER51020 Phos-tag (TM) Acrylamide,FUJIFILM
Wako
,Cat. #
304–93521
"
Amino Acid Mixture, Complete
",
Promega
,Cat. #
L4461
m7G(5′)ppp(5′)G RNA Cap Structure Analog
,
NEB
,Cat. #
S1404S
SUPERase•In RNase Inhibitor,Invitrogen,Cat.
(6)
NusG-mediated Coupling of Transcription and Translation Enhances Gene Expression by Suppressing RNA Polymerase Backtracking
Journal of Molecular Biology
January 1, 2022
Elizabeth J. Bailey, Max E. Gottesman, Ruben L. Gonzalez
the reaction; 5 μL of a solution that is 1 mM in each of the 20 essential amino acids (
Promega
, No.
L4461
); 20 μl of Promega S30 Premix without Amino Acids (No. L512A-C); vS30 μL of S30 cell extract, where
(7)
Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro
RNA
December 1, 2021
Brandon M. Trainor, Dimitri G. Pestov, Natalia Shcherbik
solution of 20 essential amino acids, complete and minus Methionine and Cysteine, were from
Promega
(cat#
L4461
and
L5511
, respectively); Ambion
mMESSAGE mMACHINE T7 Transcription kit
was purchased from
Thermo Fisher Scientific
(8)
Small Extracellular Vesicles Propagate the Inflammatory Response After Trauma
Advanced Science
December 1, 2021
Tanja Seibold et al.
reticulocyte
lysate system (
Promega
, #
L4960
) and non‐labeled complete amino acid mixture (
Promega
, #
L4461
) according to the manufacturer's description. After the translation reaction, samples were supplemented
(9)
5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
Science Advances
November 19, 2021
Luxi Chen et al.
reaction was assembled as follows: 9.9 μl of RRL, 0.3 μl of 1 mM complete amino acid mixture (
Promega
,
L4461
), 0.42 μl of 3 M KCl, 0.39 μl of RNase inhibitor, 1 μl of GFP mRNA (500 ng/μl), 0.5 μl of purified NLS-2xFlag-mCherry-HisTag
(10)
The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acids
The Journal of biological chemistry
November 1, 2021
Miles RR, Amin PH, Diaz MB, Misra J, Aukerman E, Das A, Ghosh N, Guith T, Knierman MD, Roy S,
et al
.
of 1000 V/cm. A
calibration
curve was prepared using a sample containing all 20 amino acids (
Promega
L4461
). The amino acid standard was diluted
1:100
to make 10 μM standard in BGE metabolite diluent provided
(11)
The molecular basis of coupling between poly(A)-tail length and translational efficiency
eLife
July 2, 2021
Xiang K, Bartel DP.
reticulocyte
lysate or
oocyte
extract in total volume of 20 µl containing 10 µM amino acid mix (
Promega
,
L4461
), 0.5 units SUPERase•In, 10 mM creatine phosphate (
Roche
10621714001
), 200 µg/ml creatine kinase (
Roche
,
(12)
Selective inhibition of human translation termination by a drug-like compound
Nature Communications
December 1, 2020
Wenfei Li, Stacey Tsai-Lan Chang, Fred. R. Ward, Jamie H. D. Cate
spermidine, 0.6 mM putrescine, 2 mM
TCEP
(tris(2-carboxyethyl)phosphine), 10 μM amino acid mixture (
Promega
,
L4461
), 1 U μL−1 murine RNase inhibitor (
NEB
,
M0314L
), 600 ng of mRNA, and 50 μM PF846 in 1% DMSO as control.
(13)
A broad-spectrum antiviral molecule, QL47, selectively inhibits eukaryotic translation
Journal of Biological Chemistry
February 1, 2020
Mélissanne de Wispelaere et al.
phosphokinase (Sigma, 10127566001), 2 mm DTT, 2.5 mm Mg(OAc)2, 100 mm K(OAc), 8 μm amino acids (
Promega
,
L4461
), 255 μm spermidine, and 1000 units/ml murine RNase inhibitor. Small molecules were added to the reaction
(14)
A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation
ACS Synthetic Biology
April 19, 2019
Huixin Lu, Mostafizur Mazumder, Anna S. I. Jaikaran, Anil Kumar, Eric K. Leis, Xiuling Xu, Michael Altmann, Alan Cochrane, G. Andrew Woolley
0.6 mM
GTP
, 150 mM creatine phosphate (
Roche
SKU
10621714001
), 0.24 mM 20 amino acid mix (
Promega
SKU
L4461
), and 10.2 mM DTT. In the dark, the pre-mix was split into 4 equal aliquots of 24 μL . Each aliquot
(15)
Multiple prebiotic metals mediate translation
Proceedings of the National Academy of Sciences
November 27, 2018
Marcus S. Bray, Timothy K. Lenz, Jay William Haynes, Jessica C. Bowman, Anton S. Petrov, Amit R. Reddi, Nicholas V. Hud, Loren Dean Williams, Jennifer B. Glass
from the PURExpress Δ
Ribosome
Kit (
E3313S
;
New England Biolabs
), 0.1 mM amino acid mix (catalog #
L4461
;
Promega
), Bray et al. and 0.2 mM tRNAs from
E. coli
MRE 600 (product # TRNAMRE-RO; SigmaAldrich).
(16)
Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes
RNA
July 1, 2018
Sarah E. Fritz, Nazmul Haque, J. Robert Hogg
Roche
), 0.8 mM
ATP
(
P0756S
;
New England BioLabs
), 0.1 mM spermidine, 0.1 mM complete amino acid mix (
L4461
;
Promega
), 135 mM potassium acetate (for purified mRNPs) or 50 mM potassium acetate (for IVT mRNA and
(17)
Generation of ribosome imprinted polymers for sensitive detection of translational responses
Scientific reports
July 26, 2017
King HA, El-Sharif HF, Matia-González AM, Iadevaia V, Fowotade A, Reddy SM, Gerber AP.
phosphate (Merck Millipore #2380), 1 mg/ml
yeast
tRNA (Sigma #R4752), 2 µl amino acids mix (
Promega
#
L4461
), 0.5 µl RNasin (Promega #
N2615
), 10 µl
reticulocyte
lysate (Promega #
L4151
), and 0.5 µM CHX (Sigma
(18)
Surface-enhanced Raman spectroscopy competitive binding biosensor development utilizing surface modification of silver nanocubes and a citrulline aptamer
Journal of Biomedical Optics
July 21, 2017
Brian M. Walton, George W. Jackson, Nicolaas Deutz, Gerard Cote
odd-numbered rounds, the library was first exposed to a cocktail of the 20 essential amino acids (
Promega
, catalog #
L4461
) followed by citrulline elution in phosphate-buffered saline (PBS) buffer, pH 7.4, 1 mM MgCl2 , 0.05%
(19)
Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clusters
Science Advances
June 21, 2017
Kobe C. Yuen, Brian D. Slaughter, Jennifer L. Gerton
mixed with 2 μl of TnT Reaction Buffer, 1 μl of TnT RNA Polymerase, 2 μl of Complete Amino Acid Mix (
L4461
,
Promega
), 1 μl of
RNasin Ribonuclease Inhibitor
(
N2511
,
Promega
), 2 μl of DNA plasmid, and 17 μl of
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Amino Acid Mixture, Complete from Promega
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