AhdI from New England Biolabs

Product Specs
ItemAhdI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0584S
Quantity1000 units
Concentration4 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GACNNN/NNGTC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed RepairCurrent Issues in Molecular BiologyApril 12, 2022Wanqing Xu et al.(molar ratio of Cas9:sgRNA:donor was 1:1:2, with a total of 2 μg). Reporter was digested with AhdI (R0584S, New England Biolabs) before transfection. The mixture was transfected into PFF cells using the Neon™ Transfection System

(2) An efficient and versatile CRISPR-Cas9 system for genetic manipulation of multi-drug resistant Klebsiella pneumoniaeSTAR ProtocolsMarch 19, 2021Thomas H. McConville, Marla J. Giddins, Anne-Catrin UhlemannM0530S Taq DNA polymerase with standard Taq buffer,NEB,Cat# M0273S AhdI restriction enzyme,NEB,Cat# R0584S BsaI HF V2 restriction enzyme,NEB,Cat# R3733S AarI restriction enzyme,Thermo Fisher,Cat# ER1581 XbaI

(3) Sequence and Nuclease Requirements for Breakage and Healing of a Structure-Forming (AT)n Sequence within Fragile Site FRA16DCell ReportsApril 23, 2019Simran Kaushal et al.Peptides, and Recombinant Proteins",, EcoRI-HF,NEB,Cat# R3101S BamHI-HF,NEB,Cat# R3136S AhdI,NEB,Cat# R0584S XbaI,NEB,Cat# R0145S S1 nuclease,Thermo Fisher,Cat# EN0321 Eco53kI,NEB,Cat# R0116S Polyethylene

(4) PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosisNature CommunicationsDecember 8, 2015Christian F. Nielsen et al.

(5) Intestinal epithelial suppressor of cytokine signaling 3 enhances microbial-induced inflammatory tumor necrosis factor-α, contributing to epithelial barrier dysfunctionAmerican journal of physiology. Gastrointestinal and liver physiologyJanuary 1, 2015Thagia I, Shaw EJ, Smith E, Else KJ, Rigby RJ.resistance cassette, was purchased from Epoch Life Science. Plasmids were linearized with Ahd1 (NEB no. R0584S) prior to ethanol precipitation. For transfection, cells were seeded at a concentration of 1 × 106/ml

(6) The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosineNature ProtocolsOctober 1, 2012William A Pastor, Yun Huang, Hope R Henderson, Suneet Agarwal, Anjana Raoβ-glucosyltransferase (T4-BGT; New England Biolabs, cat. no. M0357S) • AhdI (New England Biolabs, cat. no. R0584S) • FspI (New England Biolabs, cat. no. R0135S) • HindIII (New England Biolabs, cat. no. R0104S) • NdeI

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4/17/2018Plasmid Restriction Mapping Using AhdI Enzyme Triveni MahendrappaI have constructed a plasmid of size around 7.2 Kb and transformed into appropriate E. coli cells. Then plasmid prepped form these cells were used for diagnostic restriction mapping using NEB's AhdI ... Read Review

AhdI

AhdI from New England Biolabs

Sequence

New England Biolabs

Citations (6)

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AhdI from New England Biolabs

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