Anti-MBP Magnetic Beads from New England Biolabs

Product Specs
ItemAnti-MBP Magnetic Beads
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE8037S
CapacityMBP-paramyosin fusion protein 5 µg
Quantity10 mg
Particle Size1 µm
Matrixnonporous superparamagnetic bead
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Product TypeResins, Magnetic Beads
ModeAffinity Purification
TypeMagnetic Beads
Temperature4 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cryo-EM structure of the agonist-bound Hsp90-XAP2-AHR cytosolic complexNat CommunNovember 16, 2022Jakub Gruszczyk, LoÃ¯c Grandvuillemin, Josephine Lai-Kee-Him, Matteo Paloni, Christos G. Savva, Pierre Germain, Marina Grimaldi, Abdelhay Boulahtouf, Hok-Sau Kwong, Julien Bous, et al.min. 200 μl of the resulting supernatants were transferred into a new tube, mixed with 25 μl of 50% anti-MBP Magnetic beads (New England Biolabs) and agitated for 1 h. The beads were washed three times with 20 beads volume of

(2) Recognition of HIV-1 capsid by PQBP1 licenses an innate immune sensing of nascent HIV-1 DNAMolecular CellAugust 1, 2022Sunnie M. Yoh, João I. Mamede, Derrick Lau, Narae Ahn, Maria T. Sánchez-Aparicio, Joshua Temple, Andrew Tuckwell, Nina V. Fuchs, Gianguido C. Cianci, Laura Riva, et al.(Sigma Aldrich F1804), p24 (AIDS Reagent 71–31). Anti-cGAMP [PF-07043030 Pfizer (Hall et al., 2017)], Anti-MBP magnetic beads (NEB). Plasmids, siRNAs and qRT-PCR Human LentiORF cDNA clones of PQBP1 (Open Biosystems), both wild-type

(3) Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenomeNature Cell BiologyJuly 4, 2022Yingying Meng et al.mouse lung genomic DNA. His-MBP-ZBTB43 protein was pre-incubated with MBP-magnetic beads (NEB, cat. no. E8037S) at 4 °C for 90 min. To capture the ZBTB43 fraction, the fragmented mouse genomic DNA was mixed with

(4) Chemical induction of leaf senescence and powdery mildew resistance involves ethylene-mediated chlorophyll degradation and ROS metabolism in cucumberHorticulture ResearchMay 17, 2022Dingyu Zhang, Shengdong Wu, Ning Li, Jiong Gao, Shihui Liu, Shuai Zhu, Zilin Li, Guodong Ren, Benke Kuaithe pMAL-c5g vector) were expressed in Escherichia coli Rosetta (DE3). Proteins were purified with Anti-MBP Magnetic Beads (NEB). EMSA was performed using a LightShift EMSA Optimization and Control Kit (ThermoFisher Scientific)

(5) Distinct pathways of homologous recombination controlled by the SWS1–SWSAP1–SPIDR complexNature CommunicationsDecember 1, 2021Rohit Prakash et al.37 °C in a rotary shaker (600 rpm). Next, the equivalent of 5 µL of magnetic bead slurry (NEB Cat #E8037S, anti-MBP antibody coupled to superparamagnetic beads) passivated with buffer B was added to each reaction

(6) FIP200 restricts RNA virus infection by facilitating RIG-I activationCommunications biologyJuly 29, 2021Wang L, Song K, Hao W, Wu Y, Patil G, Hua F, Sun Y, Huang C, Ritchey J, Jones C, et al.and the MBP pull-down assays were performed using the anti-MBP Magnetic Beads New England Biolabs, # E8037S). FLAG-tagged proteins were purified by using the EZview Red Anti-FLAG M2 Affinity Gel (Sigma, # F2426).

(7) EndoVIPER‐seq for Improved Detection of A‐to‐I Editing Sites in Cellular RNACurrent Protocols in Chemical BiologyJune 1, 2020Steve D. Knutson, Jennifer M. HeemstraRNasin® Plus RNase Inhibitor (Promega, cat. No. N2611) Anti-MBP Magnetic Beads (New England Biolabs, cat. No. E8037S) Costar® Spin-X® Centrifuge Tube Filters, 0.22 μm pore, cellulose acetate membrane (Corning, cat. No.

(8) Transcriptional repression of GIF1 by the KIX-PPD-MYC repressor complex controls seed size in ArabidopsisNature communicationsApril 15, 2020Liu Z, Li N, Zhang Y, Li Y.His-KIX8/9 proteins were incubated at 4 °C for 1 h and pulled down with anti-MBP agarose beads (NEB, E8037s) or Ni-NTA agarose beads (Invitrogen, R90115). Beads were washed 4 times with the solution containing

(9) Chromatin structure and its chemical modifications regulate the ubiquitin ligase substrate selectivity of UHRF1Proceedings of the National Academy of SciencesAugust 28, 2018Robert M. Vaughan, Bradley M. Dickson, Matthew F. Whelihan, Andrea L. Johnstone, Evan M. Cornett, Marcus A. Cheek, Christine A. Ausherman, Martis W. Cowles, Zu-Wen Sun, Scott B. Rothbartubiquitinated nucleosomes by DNMT1 (SI Appendix, Fig. S3D), 5 pmol of MBP-DNMT1 was bound to 5 µL of anti-MBP magnetic beads (New England BioLabs), and the remainder of the procedure was identical to the previously described

(10) The Anti-activator QslA Negatively Regulates Phenazine-1-Carboxylic Acid Biosynthesis by Interacting With the Quorum Sensing Regulator MvfR in the Rhizobacterium Pseudomonas aeruginosa Strain PA1201Frontiers in MicrobiologyJuly 25, 2018Yun-Ling Fang, Bo Chen, Lian Zhou, Zi-Jing Jin, Shuang Sun, Ya-Wen Hecontrol. The immunoprecipitation reaction was initiated by the addition of anti-MBP magnetic beads (Cat # E8037S; New England Biolabs, Inc.), to each of the remaining samples. After incubation at 4°C overnight, beads

(11) A Single-Domain Response Regulator Functions as an Integrating Hub To Coordinate General Stress Response and Development in AlphaproteobacteriamBioJuly 5, 2018C. Lori, A. Kaczmarczyk, I. de Jong, U. Jenalprephosphorylated for 1 h. Twenty-five microliters of anti-MBP magnetic beads (New England Biolabs; E8037S) was added and incubated for 1 h. Beads were then concentrated using a magnet. Hexokinase and glucose

(12) The Nogo-B receptor promotes Ras plasma membrane localization and activationOncogeneJune 15, 2017Baofeng Zhao, Wenquan Hu, Suresh Kumar, Patrick Gonyo, Ujala Rana, Zhong Liu, Bei Wang, William Q. Duong, Zhe Yang, Carol L. Williams, et al.the cysteine of CAAX box) or farnesylated Ras (H-Ras-Farn). The complex was immunoprecipitated using anti-MBP magnetic beads (New England BioLabs). (D) Blocking prenyl-dependent interactions of H-Ras diminishes its interactions

(13) DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrityCell death & diseaseJuly 28, 2016Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, Jin M, Wang B, Zhang Y, Zhang Y, et al.incubated at 4 °C with GST-Ku80 proteins overnight. MBP-tagged proteins were captured by incubation with anti-MBP magnetic beads (New England Biolabs, Ipswich, MA, USA) at 4 °C for 3 h. The bead pellet was washed five times with

(14) DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in RicePLOS GeneticsOctober 23, 2015Long-Gang Cui, Jun-Xiang Shan, Min Shi, Ji-Ping Gao, Hong-Xuan LinBugBuster Protein Extraction Reagents (Novagen) and centrifuged. The supernatant was incubated with Anti-MBP Magnetic Beads (NEB) for 30 min at 4°C and washed five times with MBP column buffer. Then, supernatants containing

(15) Understanding the role of the Q338H MUTYH variant in oxidative damage repairNucleic acids researchApril 1, 2013Turco E, Ventura I, Minoprio A, Russo MT, Torreri P, Degan P, Molatore S, Ranzani GN, Bignami M, Mazzei F.HEPES–KOH 10 mM, pH 7.4, KCl 100 mM and MgCl2 10 mM buffer. The samples were then added to 30 µl of anti-MBP magnetic beads (1 mg/ml and binding capacity of 10 µg/mg) (New England BioLabs, Ipswich, MA, USA), pre-treated as described

(16) Biochemical Characterization of a Structure-Specific Resolving Enzyme from Sulfolobus islandicus Rod-Shaped Virus 2PLoS ONEAugust 17, 2011Andrew F. Gardner, Chudi Guan, William E. Jackmagnetic beads (hereafter called MBP-Hjr affinity beads) were prepared by mixing 10 µg MBP-Hjr with 0.1 mg anti-MBP magnetic beads pre-equilibrated in NEBuffer 3 (New England Biolabs). Bead complexes were mixed thoroughly and incubated

(17) A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture controlGenes & DevelopmentAugust 1, 2009Xin-Yuan Huang, Dai-Yin Chao, Ji-Ping Gao, Mei-Zhen Zhu, Min Shi, Hong-Xuan Lin(New England Biolabs). The MBPDST fusion protein was prepared as described above and purified with anti-MBP magnetic beads (New England Biolabs) according to the manufacturer’s instructions. Preparation of 6xHis-PRX fusion

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Anti-MBP Magnetic Beads from New England Biolabs

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Anti-MBP Magnetic Beads from New England Biolabs

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