Amylose Magnetic Beads from New England Biolabs

Product Specs
ItemAmylose Magnetic Beads
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE8035S
CapacityMBP-fusion protein 10 µg
Matrixsuperparamagnetic microparticles.
Particle Size10 µm
Quantity25 mg
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Product TypeResins, Magnetic Beads
ModeAffinity Purification
TypeMagnetic Beads
Temperature4 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Novel players in organogenesis and flavonoid biosynthesis in cucumber glandular trichomesPlant PhysiologyAugust 3, 2023Zhongxuan Feng, Lei Sun, Mingming Dong, Shanshan Fan, Kexin Shi, Yixin Qu, Liyan Zhu, Jinfeng Shi, Wujun Wang, Yihan Liu, et al.Escherichia coli BL21 (DE3) strain and then purified using amylose magnetic beads (New England Biolabs, Cat # E8035S). DNA gel mobility shift assay was performed using the EMSA kit (Beyotime, China, Cat # GS009) following

(2) In vitro Reconstitution of Phase-separated p62 Bodies on the Arp2/3-derived Actin NetworkBIO-PROTOCOLApril 20, 2023Tong Liu, Mengbo Xu, Na Mi10902096)Polyethyleneimines (PEI) (Polysciences, catalog number: 23966-2)Amylose beads (New England Biolabs, catalog number: E8035S)LB broth powder (Sangon Biotech, catalog number: A507002)Isopropyl-beta-D-thiogalactopyranoside (IPTG)

(3) Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)The Plant cellMarch 29, 2023Yue P, Jiang Z, Sun Q, Wei R, Yin Y, Xie Z, Larkin RM, Ye J, Chai L, Deng X.described by Li et al. (2017b). The CsMYC2-MBP fusion protein was bound to MBP magnetic beads (catalog no. E8035S; New England Biolabs, MA, USA) and incubated with CsMPK6-GST on ice for 1 h. Subsequent steps are described

(4) Linc-RAM promotes muscle cell differentiation via regulating glycogen phosphorylase activityCell RegenerationDecember 1, 2022Lili Zhai, Xin Wan, Rimao Wu, Xiaohua Yu, Hu Li, Ran Zhong, Dahai Zhu, Yong Zhangprotease inhibitor cocktail at 4 °C for 3 h. Then 100 μl of pretreated amylase magnetic beads (NEB, E8035S) were added and incubated for additional 1 h at 4 °C. After washes, the Linc-RAM-protein complex (RNPs)

(5) Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatinNature communicationsSeptember 1, 2022Zhang T, Yang H, Zhou Z, Bai Y, Wang J, Wang W.containing 300 mM KCl. The clarified cell lysate was first incubated with amylose agarose beads (NEB, Cat#E8035S) in T buffer containing 300 mM KCl and protease inhibitors for 2-3 h at 4 °C, then the beads were washed

(6) Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expressioniScienceAugust 19, 2022Alla V. Tsytsykova, Graham Wiley, Chuang Li, Richard C. Pelikan, Lori Garman, Francis A. Acquah, Blaine H.M. Mooers, Erdyni N. Tsitsikov, Ian F. Dunnbacterial strain. The recombinant proteins were purified using amylose magnetic beads (New England Biolabs, E8035S). DNase I footprinting of FGF3 promoter was performed as follows: a 360-bp fragment (from -276 to +84

(7) Arsenic 3 methyltransferase (AS3MT) automethylates on cysteine residues in vitroArchives of ToxicologyMay 1, 2022Sofiane Y. Mersaoui, Cynthia Guilbert, Hsiang Chou, Christelle Douillet, D. Scott Bohle, Miroslav Stýblo, Stéphane Richard, Koren K. Mannsame as previously described. Purification was conducted according to the manufacturer’s protocol (Amylose Magnetic Beads, New England Biolabs). Elution was achieved with buffer 50 mM MOPS pH 7.4, 10% glycerol, 0.3 M NaCl

(8) EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in LoquatFrontiers in Plant ScienceDecember 31, 2021Ze Peng, Man Wang, Ling Zhang, Yuanyuan Jiang, Chongbin Zhao, Muhammad Qasim Shahid, Yunlu Bai, Jingjing Hao, Jiangrong Peng, Yongshun Gao, et al.rpm (revolutions per minute) after addition of IPTG. The raw induced proteins were then purified with Amylose Magnetic Beads (NEB, United States). Electrophoretic Mobility Shift Assay (EMSA) assays were performed as previously

(9) An auxin-regulable oscillatory circuit drives the root clock in ArabidopsisScience AdvancesJanuary 1, 2021Juan Perianez-Rodriguez, Marcos Rodriguez, Marco Marconi, Estefano Bustillo-AvendaÃ±o, Guy Wachsman, Alvaro Sanchez-Corrionero, Hugues De Gernier, Javier Cabrera, Pablo Perez-Garcia, Inmaculada Gude, et al.buffer [0.1 M Hepes, 0.3 M KCl, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 1 mM Triton X-100]. Amylose Magnetic Beads (NEB) were used for the purification of MBP-ARF7 and MBP control using recommendations from the manufacturer.

(10) MRNIP is a replication fork protection factorScience advancesJuly 1, 2020Bennett LG, Wilkie AM, Antonopoulou E, Ceppi I, Sanchez A, Vernon EG, Gamble A, Myers KN, Collis SJ, Cejka P, et al.was removed, and slides were air-dried overnight before analysis/storage. In vitro MBP pulldowns Amylose magnetic beads (NEB) (100 μl) were washed three times in wash buffer [50 mM tris (pH 7.4), 1 mM DTT, 10% glycerol,

(11) S-nitrosylation of the zinc finger protein SRG1 regulates plant immunityNature CommunicationsDecember 1, 2018Beimi Cui, Qiaona Pan, David Clarke, Marisol Ochoa Villarreal, Saima Umbreen, Bo Yuan, Weixing Shan, Jihong Jiang, Gary J. LoakeRecombinant MBP-SRG1 was produced in E. coli BL21 (ED3) by adding 0.1 mM IPTG for 4 h and was purified by Amylose Magnetic Beads (NEB, UK). Recombinant protein GST-Topless was produced in E. coli BL21 (ED3) by adding 0.3 mM IPTG

(12) Mapping the Pax6 3’ untranslated region microRNA regulatory landscapeBMC GenomicsNovember 15, 2018Bridget C. Ryan, Kieran Lowe, Laura Hanson, Talveen Gil, Lauren Braun, Perry L. Howard, Robert L. Chowincubated on ice and supernatant was removed following centrifugation. 25 μl magnetic amylose beads (NEB, E8035S) per sample were blocked in lysis buffer containing 0.2 μg/μl yeast tRNA (Invitrogen, 15,401–029) and

(13) NOD is a plus end-directed motor that binds EB1 via a new microtubule tip localization sequenceThe Journal of cell biologySeptember 3, 2018Ye AA, Verma V, Maresca TJ.mixtures were then added to amylose magnetic beads (NEB) and incubated for an additional hour at 25°C. Amylose magnetic beads were preblocked with 10 mg/ml BSA for 1 h before addition of the proteins. Beads were next washed three

(14) Recruitment of ubiquitin-activating enzyme UBA1 to DNA by poly(ADP-ribose) promotes ATR signallingLife science allianceJune 1, 2018Kumbhar R, Vidal-Eychenié S, Kontopoulos DG, Larroque M, Larroque C, Basbous J, Kossida S, Ribeyre C, Constantinou A.amylose MBP-UBA1 fragments (100 nM of each) were incubated with 100 nM pADPr chains for 1 h at 4°C. Amylose magnetic beads (New England Biolabs) were added and incubated for approximately 30 min and then washed five times with

(15) MicroProtein-Mediated Recruitment of CONSTANS into a TOPLESS Trimeric Complex Represses Flowering in ArabidopsisPLOS GeneticsMarch 25, 2016Moritz Graeff, Daniel Straub, Tenai Eguen, Ulla Dolde, Vandasue Rodrigues, Ronny Brandt, Stephan Wenkelthe pDEST15 vector, and the coding sequence of CO into the pMALc2 vector. Amylose-Magnetic Beads [NEB E8035S] were used for the purification of MBP-CO. The HRP-conjugated anti-MBP antibody [NEB E8038] was used

(16) Dynamic Effects of Topoisomerase I Inhibition on R-Loops and Short Transcripts at Active PromotersPLOS ONEJanuary 19, 2016Jessica Marinello, Stefania Bertoncini, Iris Aloisi, Agnese Cristini, Guidantonio Malagoli Tagliazucchi, Mattia Forcato, Olivier Sordet, Giovanni Capranicofinally centrifuged at 4°C, 14000g for 20’. The isolation of MBP-fusion protein was performed with Amylose Magnetic Beads (New England Biolabs). In particular 100 μl of beads suspension were equilibrated twice with 500 μl

(17) Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2Nature communicationsJanuary 8, 2016Zhou H, Wang T, Zheng T, Teng J, Chen J.E. coli (BL21 strain), and were coupled with Glutathione Sepharose 4B beads (Amersham Bioscience) or Amylose Magnetic beads (New England BioLabs) according to the manufacturer's instructions. Then, the beads were washed five

(18) Inhibitory mechanism of the Qβ lysis protein A2Molecular MicrobiologyNovember 1, 2012C. A. Reed, C. Langlais, V. Kuznetsov, R. YoungpGADT7-T as positive and negative controls, respectively. Batch affinity fractionation experiments Amylose magnetic beads were chosen for batch affinity fractionation experiments since MBP binds specifically to amylose resin.

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