AfeI from New England Biolabs

Product Specs
ItemAfeI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0652S
Concentration10,000 units/ml
Quantity200 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

AGC^GCT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Broad-spectrum humanized monoclonal neutralizing antibody against SARS-CoV-2 variants, including the Omicron variantFrontiers in Cellular and Infection MicrobiologyAugust 14, 2023Kun Wen, Jian-Piao Cai, Xiaodi Fan, Xiaojuan Zhang, Cuiting Luo, Kai-Ming Tang, Huiping Shuai, Lin-Lei Chen, Ricky Ruiqi Zhang, Jianwen Situ, et al.expression vector with restriction endonucleases AfeI and NheI (New England Biolabs, Ipswich, MA. USA; Cat# R0652L and R3131L) for the heavy chain, and AfeI and BsiWI (New England Biolabs, Ipswich, USA; Cat# R0553L)

(2) Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxicationbioRxivAugust 3, 2023Atossa C. Ghorashi, Andrew Boucher, Stephanie A. Archer-Hartmann, Nathan B. Murray, Rohit Sai Reddy Konada, Xunzhi Zhang, Chao Xing, Parastoo Azadi, Ulf Yrlid, Jennifer J. Kohlerlentiviral vector (Addgene, plasmid no. 52962) cut with BamHI-HF and AgeFI (NEB, catalog no. R3136S and R0652S, respectively) according to manufacturer’s guidelines. Plasmids were transformed into DH5α competent

(3) Synthesis and cloning of long repeat sequences using single-stranded circular DNAFrontiers in Bioengineering and BiotechnologyMarch 9, 2023Afsana Bhuiyan, Shuichi Asakawagenetics co. FG-91302). The pIRES2 DsRed-Express2 vector (Takara Bio, 632540) was digested by AfeI (NEB R0652) and Shrimp Alkaline Phosphatase (rSAP) (NEB M0371) to prepare blunt ended vector. pIRES and the purified

(4) SFPQ promotes RAS-mutant cancer cell growth by modulating 5′-UTR mediated translational control of CK1αNAR CancerSeptember 27, 2022Venetia Jing Tong Kok et al.was generated by digesting the CK1α 5′-UTR psiCHECK™-2 constructs with restriction enzyme AfeI (NEB, R0652S) in CutSmart® buffer for 1 h at 37°C. Following which, in-vitro transcription was performed with T7

(5) Sensitive and reproducible cell-free methylome quantification with synthetic spike-in controlsCell Reports MethodsSeptember 19, 2022Samantha L. Wilson, Shu Yi Shen, Lauren Harmon, Justin M. Burgener, Tim Triche, Scott V. Bratman, Daniel D. De Carvalho, Michael M. Hoffman"New England Biolabs, Ipswitch, MA, USA",Cat# R171S AfeI,"New England Biolabs, Ipswitch, MA, USA",Cat# R0652S ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Mycoplasma-free

(6) Rapid detection of SARS-CoV-2 RNA in saliva via Cas13Nature Biomedical EngineeringAugust 11, 2022Sita S. Chandrasekaran, Shreeya Agrawal, Alison Fanton, Aditya R. Jangid, Bérénice Charrez, Arturo M. Escajeda, Sungmin Son, Roger Mcintosh, Huyen Tran, Abdul Bhuiya, et al.digestion of rLAMP DNA products was performed on 3 μl of amplified rLAMP product using 2 μl of AfeI (NEB R0652L), 2 μl 10× CutSmart Buffer and 13 μl of water. This 20 μl of reaction was heated at 37 °C for 30 min.

(7) miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell levelEMBO reportsApril 5, 2022Wang D, Xiu J, Zhao J, Luo J.enzyme,NEB,R3136V EcoRI‐HF restriction enzyme,NEB,R3101V XhoI restriction enzyme,NEB,R0146V AfeI restriction enzyme,NEB,R0652S ClaI restriction enzyme,NEB,R0197V KpnI‐HF restriction enzyme,NEB,R3142V PacI restriction enzyme,NEB,R0547S PmeI

(8) Chondroitin 6-sulphate is required for neuroplasticity and memory in ageingMolecular PsychiatryOctober 1, 2021Sujeong Yang et al.AGCGCTGGCCGGCCGTTTAAAC 3′ and was cloned into plasmid AAV-PGK-Cre (Addgene plasmid # 24593) between the AfeI (NEB, R0652) and EcoRI (NEB, R0101) sites to substitute the Cre recombinase gene. The eGFP sequence of AAV-CMV-eGFP

(9) DNA length tunes the fluidity of DNA-based condensatesBiophysical journalApril 6, 2021Muzzopappa F, Hertzog M, Erdel F.different sizes (Fig. S1, A–C), 1.5 μg of labeled λ-DNA was incubated at 37°C for 2 h with 1) AfeI (NEB, R0652); 2) BamHI (NEB, R3136) and KpnI (NEB, R0142); 3) AatII (NEB, R0117), BamHI (NEB, R3136), and KpnI (NEB,

(10) IQGAP1 Negatively Regulates HIV-1 Gag Trafficking and Virion ProductionCell reportsMarch 24, 2020Sabo Y, de Los Santos K, Goff SP.experiments that used pEGFP-IQGAP1 was generated by digesting pEGFP-C1 with AfeI and SmaI (NEB; Cat# R0652 and R0141), gel purification with QIAGEN QIAquick Gel Extraction Kit and self-ligation of the digested

(11) Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing systemScientific ReportsDecember 1, 2019Jeong Gu Kang, Jin Suk Park, Jeong-Heosn Ko, Yong-Sam Kimdinucleotides and reporter sites was cloned into the above donor pUC19 vector following digestion with AfeI (R0652L, New England Biolabs) and BamHI (R0136S, New England Biolabs). The dCas9-Tet1 plasmid was constructed

(12) Reporter-ChIP-nexus reveals strong contribution of the Drosophila initiator sequence to RNA polymerase pausingeLifeApril 25, 2019Shao W, Alcantara SG, Zeitlinger J.kit,recJ,New England Biolabs,#M0264L, Commercial assay or kit,Restriction emzyme AfeI,New England Biolabs,#R0652S, Commercial assay or kit,Restriction emzyme EcoRV-HF,New England Biolabs,#R3195S, Commercial assay

(13) High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cellsScientific reportsOctober 11, 2018Idoko-Akoh A, Taylor L, Sang HM, McGrew MJ.DNA with 10 units of restriction enzyme (EcoRI-HF (NEB #R3101S), BamHI-HF (NEB #R3136S), AfeI (NEB #R0652S), AatII (NEB #R0117S)) in CutSmart buffer (NEB) incubated at 37 °C for 1 h. The digestion products were

(14) A Transcription Activator-Like Effector (TALE) Toolbox for Genome EngineeringNature ProtocolsJanuary 5, 2012Neville E. Sanjana, Le Cong, Yang Zhou, Margaret M. Cunniff, Guoping Feng, Feng Zhangno. ER0451) BsaI-HF (New England Biolabs, cat. no. R3535L) AfeI (New England Biolabs, cat. no. R0652S) Fermentas Tango Buffer and 10x NEBuffer 4 (included with enzymes) 100x Bovine Serum Albumin (New England Biolabs

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AfeI

AfeI from New England Biolabs

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New England Biolabs

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AfeI from New England Biolabs

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