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AciI
AciI
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AciI
AciI from New England Biolabs
Be the first to write a review!
Citations:
(22)
Supplier Page
Supplier Page from
New England Biolabs for
AciI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
AciI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0551L
Concentration
5 u/µL
Quantity
1000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
C/CGC + G/CGG
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (22)
(1)
Eliminating Malaria Vectors with Precision Guided Sterile Males
bioRxiv
July 26, 2023
Andrea L. Smidler, Reema A. Apte, James J. Pai, Martha L. Chow, Sanle Chen, Agastya Mondal, Héctor M. Sánchez C., Igor Antoshechkin, John M. Marshall, Omar S. Akbari
1114A.S41 (5’ CGGAAAGTTTATCATCCACTCTGAC 3’) primers, and the amplicon was digested with Acil (
NEB
cat#
R0551S
). To identify mutations by gRNAfle.7, the fle locus was amplified with the 1154A.S23 (5’ CTCAGCAAGCAGTATGCCAAC
(2)
Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
Journal of Developmental Biology
June 16, 2023
Timothy Erickson, William Paul Biggers, Kevin Williams, Shyanne E. Butland, Alexandra Venuto
Beverly, MA, USA, 2302830), and performing a NaCl-isopropanol precipitation with glycogen (
Thermo Fisher
,
R0551
) acting as an inert carrier. Biotinylated RNA was fragmented to approximately 200–500 bases using the
(3)
Toll-like receptor 2-mediated downstream cytokine levels as determinant of malaria pathogenesis
Indian Journal of Medical Research
June 7, 2023
Manash Pratim Kalita, Tarun Kumar Basumatary, Subhash Medhi, Ramie Husneara Begum
agarose gel. The
PCR
products (14 µl) were then digested with the restriction enzyme
AciI
(Cat. No-
R0551S
,
New England Biolabs
, USA) at 37°C, incubated overnight, and were then analyzed based on band pattern
(4)
Induced hepatic stem cells maintain self-renewal through the high expression of Myc coregulated by TET1 and CTCF
Cell & Bioscience
September 2, 2022
Chen Wang, Xinlu Yu, Sai Ding, Yang Liu, Hongxia Zhang, Jingbo Fu, Bing Yu, Haiying Zhu
restriction endonuclease-
PCR
(MSRE-
PCR
) The genomic DNA extracted from cells was digested with
AciI
(
R0551
V,
New England BioLabs
) and
HpaII
(
R0171
V, New England BioLab), respectively. Genomic DNA digested
(5)
Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection
Life Science Alliance
May 1, 2022
Martin Scherzer, Fosco Giordano, Maria Solé Ferran, Lena Ström
with 80% EtOH, and resuspended in 50 μl of TE. 10 μl of each sample were digested with 10 U of
AciI
(
R0551S
;
New England Biolabs
) and 10 U
MseI
(
R0525S
;
New England Biolabs
) in a total reaction volume of 30 μl
(6)
Mistranslation Reduces Mutation Load in Evolving Proteins through Negative Epistasis with DNA Mutations
Molecular Biology and Evolution
October 27, 2021
Jia Zheng, Ning Guo, Andreas Wagner
°C overnight. After that, we precipitated the
ligation
product by adding 80 µl ddH2O, 1 µl glycogen (
R0551
,
Thermo Scientific
), 50 µl 7.5 M ammonium acetate (A2706-100ML, Sigma), and 375 µl ice-cold absolute
(7)
DNA methylation patterns in juvenile systemic sclerosis and localized scleroderma
Clinical Immunology
July 1, 2021
Patrick Coit, Kaila L. Schollaert, Emily M. Mirizio, Kathryn S. Torok, Amr H. Sawalha
To confirm proper CpG methylation, 200ng of plasmids were double digested with
AciI
(5’-C/CGC-3’) (
R0551S
;
New England BioLabs
; Ipswich, MA, USA), which is blocked by methylated CpG sites, and
SacI-HF
(5’-GAGCT/C-3’)
(8)
Cis-Repression of Foxq1 Expression Affects Foxf2-Mediated Gene Expression in Palate Development
Frontiers in Cell and Developmental Biology
April 8, 2021
Jingyue Xu, Han Liu, Yu Lan, Rulang Jiang
amplified from wildtype, Foxf2+/–, and Foxf2–/– cDNA samples were digested overnight using
AciI
(
NEB
,
R0551L
) at 37oC. Quantification of the digested and undigested DNA fragments was performed by
QIAxcel Advanced
(9)
DNA length tunes the fluidity of DNA-based condensates
Biophysical journal
April 6, 2021
Muzzopappa F, Hertzog M, Erdel F.
KpnI
(
NEB
, R0142); 3)
AatII
(
NEB
, R0117),
BamHI
(NEB,
R3136
), and
KpnI
(NEB, R0142); or 4)
AciI
(
NEB
,
R0551
),
HindIII
(
NEB
,
R0104
), and
DraI
(
NEB
,
R0129
). The 1 kb and 5 kb fragments were generated by
PCR
using
(10)
High-throughput genotyping assays for identification of glycophorin B deletion variants in population studies
Experimental Biology and Medicine
April 1, 2021
Dominic SY Amuzu et al.
and 3b, with primers purchased from IDT (Leuven, Belgium). Restriction enzymes
AciI
(Catalog number:
R0551L
,
NEB
, UK) and
BsrBI
(Catalog number:
R0102L
,
NEB
, UK) were purchased (Table 4a). Reactions were prepared
(11)
Tetracyclines promote survival and fitness in mitochondrial disease models
Nature metabolism
January 1, 2021
Perry EA, Bennett CF, Luo C, Balsa E, Jedrychowski M, O'Malley KE, Latorre-Muro P, Ladley RP, Reda K, Wright PM,
et al
.
(
New England Biolabs
,
R0114S
) and ND1 and control cybrids were digested with Acil (
New England Biolabs
,
R0551S
). The reactions were incubated for 1 hour at 37°C and run on a 2% agarose gel. Oxygen consumption measurements. For
(12)
Epigenome-wide base-resolution profiling of DNA methylation in chorionic villi of fetuses with Down syndrome by methyl-capture sequencing
Clinical Epigenetics
December 1, 2019
Ji Hyae Lim, Yu-Jung Kang, Bom Yi Lee, You Jung Han, Jin Hoon Chung, Moon Young Kim, Min Hyoung Kim, Jin Woo Kim, Youl-Hee Cho, Hyun Mee Ryu
included (normal DS = 5:4). DNA samples (10 ng) were treated with
AciI
(
New England Biolabs
, catalog no.
R0551L
) as the methylation-specific restriction enzyme (MSRE) according to the manufacturer’s instructions,
(13)
Lead, Cadmium and Zinc Phytotoxicity Alter DNA Methylation Levels to Confer Heavy Metal Tolerance in Wheat
International Journal of Molecular Sciences
September 20, 2019
Sarfraz Shafiq, Qudsia Zeb, Asim Ali, Yasar Sajjad, Rashid Nazir, Emilie Widemann, Liangyu Liu
method from
wheat
roots after 48 h of Pb, Cd and Zn treatments. Then the DNA was digested with
AciI
(
R0551S
New England Biolabs
, Ipswich, MA, USA) and
hpaII
(
R0171S
New England Biolabs
, Ipswich, MA, USA) for
(14)
Kat2a and Kat2b Acetyltransferase Activity Regulates Craniofacial Cartilage and Bone Differentiation in Zebrafish and Mice
Journal of Developmental Biology
November 12, 2018
Rwik Sen, Sofia A. Pezoa, Lomeli Carpio Shull, Laura Hernandez-Lagunas, Lee A. Niswander, Kristin Bruk Artinger
and (Rv) 50 -GCGTTTCTCAGGTGGTAGTT-30 , followed by digestion with
AciI
(
New England BioLabs
, Catalog#
R0551L
), yields a digested lower band for mutant, an undigested upper band for WT, and a combination of both
(15)
Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs
Developmental Cell
September 1, 2018
John Wang, Yandong Yin, Stephanie Lau, Jagadish Sankaran, Eli Rothenberg, Thorsten Wohland, Martin Meier-Schellersheim, Holger Knaut
,
Thermo Fisher
,
A33082
BsrI
,
New England Biolabs
,
R0527S
Hpych4v
,
New England Biolabs
,
R0620L
AciI
,
New England Biolabs
,
R0551S
ApoI
,
New England Biolabs
,
R0566L
Taqαi,
New England Biolabs
,
R0149L
Brefeldin A,
Sigma Aldrich
,
B7651
–5MG Critical
(16)
Comprehensive Whole DNA Methylome Analysis by Integrating MeDIP-seq and MRE-seq
Methods in Molecular Biology
June 11, 2018
Xiaoyun Xing, Bo Zhang, Daofeng Li, Ting Wang
enzymes for DNA digestion.
HpaII
(
NEB
; cat. no.
R0171S
).
HinP1I
(
NEB
; cat. no.
R0124S
).
AciI
(
NEB
; cat. no.
R0551S
).
HpyCH4IV
(
NEB
; cat. no.
R0619S
).Bsh1236I (
Thermo Scientific
; cat. no.
ER0921
).
Gel electrophoresis
(17)
Biological control of Sirex noctilio (Hymenoptera: Siricidae) in the northeastern United States using an exotic parasitic nematode
Biological Control
April 1, 2017
David W. Williams, Ann E. Hajek
(18)
Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating
Human Mutation
October 1, 2016
Xin-Ming Shen, Tatsuya Okuno, Margherita Milone, Kenji Otsuka, Koji Takahashi, Hirofumi Komaki, Elizabeth Giles, Kinji Ohno, Andrew G. Engel
5′-ATCGTGCCCTGTGTGCTCATC-3′ on exon 7 and 5′-CCACCCAGCGTCCGAATAAA-3′ on intron 8 by
PCR
, and was digested with
AciI
(
R0551
,
New England Biolabs
).
AciI
digestion gave rise to two fragments of 53 and 45 bp from the mutant allele,
(19)
Association of variants in BAFF (rs9514828 and rs1041569) and BAFF-R (rs61756766) genes with the risk of chronic lymphocytic leukemia
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
October 1, 2016
Jasek M, Bojarska-Junak A, Wagner M, Sobczyński M, Wołowiec D, Roliński J, Karabon L, Kuśnierczyk P.
of
assays
used in this study). The BAFF rs9514828 was examined by both RFLP [16] (
AciI
, catalog no.
R0551
,
New England Biolabs
® Inc., Ipswich, MA, USA) and TaqMan methods (
assay
ID C_29641742_10) or by double
(20)
Sex differences in Gadd45b expression and methylation in the developing rodent amygdala
Brain Research
July 1, 2016
Stacey L. Kigar, Liza Chang, Margaret R. Hayne, Nicolette T. Karls, Anthony P. Auger
rat
was divided equally into two tubes and digested with either
AciI
(
New England Biolabs
; catalog #
R0551
) or
ClaI
(
New England Biolabs
; catalog #
R0197
) in the same buffer conditions at 37°C for 90 min. Enzymes
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AciI from New England Biolabs
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