Fig 1: Detection of mutation. (A) SSCP analysis of exon 12. The expected size is 280 bp. The numbers above the lanes denote the sample number. M stands for DNA molecular marker (Fisher BioReagents™ exACTGene™ DNA Ladders). (B) Sequencing chromatogram. The chromatogram showed heterozygous mutation where one allele has been mutated from C to G.