Single-cell sequencing requires sophisticated methods for advanced cell partitioning, amplification of low-abundance nucleic acids, higher sequencing outputs, and data analysis of larger datasets. In return, researchers are able to uncover the nuanced differences among individual cells necessary for significant scientific discoveries. This eBook provides a comprehensive view into the essentials and optimization strategies for single-cell sequencing, along with a noteworthy case study to aid readers in effectively planning their own projects.
Topics covered in this eBook include
- Core principles of single-cell sequencing
- Key considerations for scientists planning a single-cell sequencing project
- Strategies to optimize single-cell RNA-Seq experiments by removing problematic cells
- The benefits of an alternate cell fixation method
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