PNGase F

Peptide:N-glycosidase F, also known as PNGase F, is a recombinant glycosidase with a molecular weight of 36 kDa. It cleaves the N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins, resulting in a deaminated peptide or protein and a free glycan. It is active in a wide pH range and in the presence of many reagents. It can be used for protein trafficking, characterization of glycan structure, determining locations of glycosylation in proteins and identifying whether a protein is glycosylated. It is also used for the deglycosylation of glycoproteins. Visit the enzyme supplier page for more product information.