Endonuclease VIII

Endonuclease VIII is a bacterial enzyme that functions as a DNA N-glycosylase and an abasic site lyase (AP lyase). DNA glycosylase and glycosylase/abasic (AP) lyases are involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Also known as ‘Nei’ after the gene that encodes it, the enzyme consists of two domains and several defined structural motifs. The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves the DNA phosphodiester backbone at AP sites by beta-delta elimination, creating nicks in the form of a 1-nucleotide DNA gap with 5' and 3' phosphate termini. It has been suggested that endonuclease VIII plays a role in replication-associated DNA repair. Visit the supplier page for more enzyme information.