Benzonase

Benzonase is a genetically engineered exonuclease, originally extracted from the enterobacterium Serratia marcescens. It is a homodimer in structure with a monomer molecular mass of 30 kDa and contains two stabilizing disulfide bonds. Also known as ‘Golden nuclease’, it can digest nucleic acids – both DNA and RNA – into 5'-monophosphate terminated oligonucleotides 2–5 bases in length. It has no sequence specificity but it is reported to exhibit a preference for G-C rich regions in double-stranded DNA. It can be used to remove fragments of nucleic acids from recombinant proteins, digest native or heat-denatured DNA and RNA, reduce viscosity caused by nucleic acids, prepare samples for electrophoresis and chromatography, and assist in the purification of viral vectors and vaccines. Visit the enzyme supplier page for more product information.