RNase-Free DNase

DNases, or deoxyribonucleases, are enzymes that hydrolyze DNA. Among these is DNase I, an endonuclease that nonspecifically cleaves both single and double-stranded DNA, chromatin and RNA:DNA hybrids. It does so by hydrolyzing phosphodiester linkages, yielding mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. The enzyme acts upon different sites on the strands depending on the coenzymes present, such as either Mg2+ or Mn2+. DNase is required in applications that require the general removal of DNA, such as during RNA purification. RNase-free DNase, which is guaranteed not to contain any ribonucleases, is used widely for cell lysis, RNA purification, transcription reactions where the DNA template is degraded, DNase I footprinting, and nick translation. DNase products are often supplied with a reaction buffer for optimal enzyme activity. Visit the enzyme supplier page for more details, such as quantity and applications.