Fig 1: Akoya Biosciences Phenocycler investigation of lymphocytes in FD compared to control. (A) A duodenal biopsy from 1x control and 1x FD patient were stained using the Akoya Biosciences Phenocycler platform (top images scale bar = 200µM, bottom images = 100µM). The expression of CD3e, CD4, CD8, CD20CD44, CD45RO, and DAPI as the nuclear marker are shown. 10x and 20x magnification. (B) Heatmap demonstrating the fold change expression of each marker in each region generated by clustering of the DAPI positive population using CytoMAP. (C) The prevalence of each clustered region in the control sample compared to the FD sample. (D) t-SNE plot of lymphocyte-associated markers (CD3e, CD4, CD8, CD20, CD44, CD45RO) in the FD patient compared to the control. (E) t-SNE plot of lymphocyte-associated markers (CD3e, CD4, CD8, CD20, CD44, CD45RO) colored by clustered region in the control and FD sample. (F) t-SNE plots of memory lymphocytes (CD45RO+CD4+/CD8+) MFI intensity in control and FD sample, (G) t-SNE plots of activated lymphocytes (CD44+CD4+/CD8+) MFI intensity in control and FD sample. Magenta = positive cells, grey = negative cells. n=1 control, n=1 FD duodenal biopsy. .