EVOS™ M7000 Imaging System from Thermo Fisher Scientific

Product Specs
ItemEVOS™ M7000 Imaging System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberAMF7000
Quantity1 system
TypeCell Imaging System
ApplicationsFluorescent and colorimetric imaging, Z-stack capability, image stitching and tiling, time-lapse imaging, and single-click multichannel capture, full automation
Light SourceLED

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Hypoxia promotes proliferation and inhibits myogenesis in broiler satellite cellsPoultry ScienceJanuary 1, 2024Usuk Jung, Minjeong Kim, Presley Dowker-Key, Simon Noë, Ahmed Bettaieb, Elizabeth Shepherd, Brynn Voycells were fixed with 4% paraformaldehyde for 10 min at RT and lipid droplets were visualized using an EVOS M7000 imaging system (Thermo Fisher Scientific, Waltham, MA). Quantification of BOPIPY 493/503 in myotubes was performed

(2) Loss of Tumour Suppressor TMEM127 Drives RET-mediated Transformation Through Disrupted Membrane DynamicsbioRxivDecember 19, 2023Timothy J. Walker, Eduardo Reyes-Alvarez, Brandy D. Hyndman, Michael G. Sugiyama, Larissa C.B. Oliveira, Aisha N. Rekab, Mathieu J.F. Crupi, Rebecca Cabral-Dias, Qianjin Guo, Patricia L.M. Dahia, et al.performed using the Duolink® PLA reagents according to the manufacturer’s instructions (Sigma Aldrich). An EVOS M7000 imaging system (ThermoFisher Scientific) with a 40x air objective was used to acquire immunofluorescent images of the

(3) Targeting S100A9 protein affects mTOR-ER stress signaling and increases venetoclax sensitivity in Acute Myeloid LeukemiaBlood Cancer JDecember 18, 2023Rong Fan, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Elke De Bruyne, Eline Menu, Nathan De Beule, Ann De Becker, Gamze Ates, Ann Massie, et al.streptomycin. Plates were incubated at 37 °C, 5% CO2 for 14 days. The colonies were photographed using the EVOS™ M7000 Imaging System (Thermo Fisher Scientific). Western blot KG-1a and MOLM-13 cells were cultured at a density of 2.5

(4) Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblastPeerJDecember 18, 2023Jiin Lee, Jeongwoo Kwon, Yu-Jin Jo, Seung-Bin Yoon, Jae-Hwan Hyeon, Beom-Jin Park, Hyeong-Ju You, Changsic Youn, Yejin Kim, Hyun Woo Choi, et al.with Hoechst 33342 (Sigma, Burlington, MA, USA), and apoptosis-positive cells were examined under the EVOS M7000 imaging system (Invitrogen, Carlsbad, CA, USA). RNA isolation and quantitative real time–PCR mRNA was isolated from

(5) Visible light-induced switching of soft matter materials properties based on thioindigo photoswitchesNat CommunDecember 14, 2023Sarah L. Walden, Phuong H. D. Nguyen, Hao-Kai Li, Xiaogang Liu, Minh T. N. Le, Loh Xian Jun, Christopher Barner-Kowollik, Vinh X. Truongincubation, hydrogels were removed, and microscopic images of cells were taken using an Invitrogen EVOS™ M7000 Imaging System. Subsequently, cells were harvested and washed in cold Annexin V binding buffer, followed by incubation

(6) Integrated systems biology approach identifies gene targets for endothelial dysfunctionMolecular Systems BiologyDecember 6, 2023Iguaracy Pinheiro‐de‐Sousa, Miriam Helena Fonseca‐Alaniz, Girolamo Giudice, Iuri Cordeiro Valadão, Silvestre Massimo Modestia, Sarah Viana Mattioli, Ricardo Rosa Junior, Lykourgos‐Panagiotis Zalmas, Yun Fang, Evangelia Petsalaki, et al.,ThermoFisher Scientific,23225 QuantiTect SYBR green PCR kit,Qiagen,204143 EVOS M7000 image system,ThermoFisher Scientific,AMF7000 ImageQuant LAS 4000 mini,GE Healthcare, QuantStudio 12 K Flex system,ThermoFisher Scientific,

(7) Sample Preparation Method for MALDI Mass Spectrometry Imaging of Fresh-Frozen SpinesAnalytical ChemistryNovember 28, 2023Kayle J. Bender, Yongheng Wang, Chuo Ying Zhai, Zoe Saenz, Aijun Wang, Elizabeth K. Neumannwas then coverslipped with glycerol wet mount medium (Rs’ Science) for microscopic imaging using an EVOS M7000 imaging system (brightfield, 20× objective; Invitrogen, Waltham, MA). Matrix Application 1,5-Diaminonaphthalene (DAN,

(8) Effects of Dickkopf-1 (DKK-1) on Prostate Cancer Growth and Bone MetastasisCellsNovember 24, 2023Shiyu Yuan, Nathan K. Hoggard, Noriko Kantake, Blake E. Hildreth, Thomas J. RosolLeukocyte (TRAP) Kit, Sigma–Aldrich, St Louis, MO, USA). Images of stained cells were taken using the EVOS M7000 Imaging System (Thermo Fisher). Osteoclasts that had more than three nuclei were considered TRAP-positive multinucleated

(9) Establishing a three-dimensional scaffold model of hepatoblastomaFrontiers in Bioengineering and BiotechnologyNovember 23, 2023Elena Johanna Weigl, Salih Demir, Tanja Schmid, Alina Hotes, Oliver Muensterer, Roland Kapplermodels were washed two times with PBS. Images of immunofluorescent stainings were captured with the EVOS™ M7000 imaging system (Invitrogen). 2.8 MTT cell viability assay Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

(10) Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot StudyDiagnostics (Basel)November 20, 2023Ekaterina Gubarkova, Arseniy Potapov, Alexander Moiseev, Elena Kiseleva, Darya Krupinova, Ksenia Shatilova, Maria Karabut, Andrey Khlopkov, Maria Loginova, Stefka Radenska-Lopovok, et al.Cambridge, UK) in order to identify lymphatic vessels. Histological sections were examined using the EVOS M7000 Imaging System (Thermo Fisher Scientific Inc., Waltham, MA, USA) in transmitted light. 2.6. Statistics The variables

(11) Dysregulated monocyte-derived macrophage response to Group B Streptococcus in newbornsFrontiers in ImmunologyNovember 14, 2023Denho Ravi, Erato Ntinopoulou, Nessim Guetta, Manuela Weier, Verena Vogel, Barbara Spellerberg, Parham Sendi, Sandrine Gremlich, Thierry Roger, Eric Giannonisteps (34). MDMs were exposed to FITC-labeled GBS and pictures of cells were taken at 1 hour with an EVOS M7000 Imaging System (ThermoFisher). Phagocytosis of FITC-labeled GBS was quantified in 100 macrophages. The viability of

(12) Combination of inhibitors for two glycolytic enzymes portrays high synergistic efficacy against Cryptosporidium parvumAntimicrobial Agents and ChemotherapyOctober 18, 2023Shahbaz M. Khan, Muhammad Rashid Bajwa, Rachael Y. Lahar, William H. Witolastained cells were washed with PBS, followed by rinsing with water, and then imaged with an inverted EVOS M7000 Imaging System (Invitrogen) using a fully automated scan protocol. The EVOS M7000 software was used to program the

(13) A Predictive and an Optimization Mathematical Model for Device Design in Cardiac Pulsed Field Ablation Using Design of ExperimentsJournal of Cardiovascular Development and DiseaseOctober 11, 2023Eoghan Dunne, Jara M. Baena-Montes, Kevin Donaghey, Cormac Clarke, Marcin J. Kraśny, Bilal Amin, Tony O’Halloran, Leo R. Quinlan, Adnan Elahi, Martin O’Halloranmonolayer was assessed two hours post-ablation using the live/dead assay by imaging monolayers on the EVOS M7000 Imaging System microscope (Life Technologies Corporation, Bothell, Washington, DC, USA). Captured images were further

(14) High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seqNature BiotechnologyOctober 1, 2023Yang Liu, Marcello DiStasio, Graham Su, Hiromitsu Asashima, Archibald Enninful, Xiaoyu Qin, Yanxiang Deng, Jungmin Nam, Fu Gao, Pino Bordignon, et al.temperature. Microscope setup The tissue image and two flow channel/tissue images were scanned with the Invitrogen EVOS M7000 imaging system using a ×10 objective. Images were taken with mono-color mode and stitched with ‘More Overlap’ settings.

(15) Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma CellsInternational journal of molecular sciencesSeptember 23, 2023Grunwald V, Ngo HD, Formanski JP, Jonas JS, Pöhlking C, Schwalbe B, Schreiber M.Technologies, Bad Wildbad, Germany). The green fluorescence of HIVgfp-infected cells was monitored using an EVOS M7000 Imaging System (Thermo Fisher Scientific, Braunschweig, Germany). 5. Conclusions Improving targeted gene transfer

(16) βPix Guanine Nucleotide Exchange Factor Regulates Regeneration of Injured Peripheral AxonsInternational journal of molecular sciencesSeptember 20, 2023Jeon Y, Shin YK, Kim H, Choi YY, Kang M, Kwon Y, Cho Y, Chi SW, Shin JE.sample group were imaged using an EVOS M7000 imaging system (Thermo Fisher Scientific, Waltham, MA, USA, AMF7000) or a charge-coupled camera device (DS-Qi2) on a fluorescence microscope (Ti-E, Nikon, Tokyo, Japan)

(17) Capture and inactivation of viral particles from bioaerosols by electrostatic precipitationiScienceSeptember 15, 2023Hannah E. Preston, Rebecca Bayliss, Nigel Temperton, Martin Mayora Neto, Jason Brewer, Alan L. Parkerexpression using a microscopic imaging system (EVOS M7000, Invitrogen™, Thermo Fisher Scientific, #AMF7000), then harvested in FACS buffer and fixed with 4% Paraformaldehyde. Flow Cytometry was performed, using

(18) Multimodal OCT Control for Early Histological Signs of Vulvar Lichen Sclerosus Recurrence after Systemic PDT: Pilot StudyInternational Journal of Molecular SciencesSeptember 12, 2023Arseniy Potapov, Lev Matveev, Alexander Moiseev, Elena Sedova, Maria Loginova, Maria Karabut, Irina Kuznetsova, Viktoriya Levchenko, Elena Grebenkina, Sergey Gamayunov, et al.lymphatic endothelial cell markers (ab236529; Abcam, Cambridge, UK). Sections were imaged using All-in-one Type EVOS M7000 Imaging System (Thermo Fisher Scientific Inc., Waltham, MA, USA). 4.5. Statistical Analysis To control early histological

(19) Microfluidic Isolation of Disseminated Tumor Cells from the Bone Marrow of Breast Cancer PatientsInternational Journal of Molecular SciencesSeptember 11, 2023Léa L. Volmer, Cansu E. Önder, Barbara Volz, Anjali R. Singh, Sara Y. Brucker, Tobias Engler, Andreas D. Hartkopf, André Kochwith 5% CO2. The culture medium was renewed every 3 days. 4.10. Imaging Imaging was performed on the EVOS M7000 Imaging system (Thermo Fisher Scientific, MA, USA). 4.11. Statistical Analysis For statistical comparison of the

(20) Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomicsSTAR ProtocolsSeptember 1, 2023Junyi Zhang, Jakob Straehle, Kevin Joseph, Nicolas Neidert, Simon Behringer, Jonathan Göldner, Andreas Vlachos, Marco Prinz, Christian Fung, Jürgen Beck, et al.Imaging,01-0224 Hamilton micro syringe,Hamilton,80330 EVOS M7000 microscope,Thermo Fisher Scientific,AMF7000 EVOS onstage incubator,Thermo Fisher Scientific,AMC1000 C-Tube,Miltenyi Biotech,130-093-237 Chromium

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