Fig 2: Histopathological examination was performed on an Olympus CX43 Biological Microscope and cellSens acquisition software. The Olympus CX43 settings were as follows: trinocular tube 2 with two widefield eyepieces for FN20, WHB10× objective in the light path, digital camera for microscope (DP22), and U-25LBD microscope polarizing filter (batch number 6,397,600). Histopathological examination with haematoxylin and eosin stain of the cystic lesion showed the characteristic ciliated pseudostratified columnar epithelium and cyst wall comprising fibrous tissue, inflammatory cells, and smooth muscle (original magnification ×100)

Fig 3: Representative images of Urine sediment analysis using automated and manual point-of care techniques for cases 1 and 2. Urine specimens from each patient were divided into two containers and assessed simultaneously. One was analyzed using the Iris iQ200 Series Automated Urine Microscopy System analyzer (Iris Diagnostics, Beckman Coulter). Representative images from the “results screen” are show in Panels A. The second aliquot of each urine specimen was evaluated by point of care microscopy. 10 ml of urine was centrifuged at 400 × g for 5 min. Microscopic inspection of the urinary sediment was performed using an Olympus CX43 microscope, 40X objective, and photographed using an attached Olympus EP50 digital camera. Representative images are seen in Panels B. 1B is brightfield without stain. The sediment in 2B was stained for 1 min using Sternheimer-Malbin stain (Globe Scientific 3810, 1 drop added to resuspended sediment). In Panel 1A, numerous red blood cells (RBC’s) seen here in the left-hand column were identified in addition to many unclassified particles which would trigger manual review by a laboratory technician. 1B shows a pigmented granular cast and numerous nondysmorphic RBC’s. Panel 2A demonstrates white blood cells (WBC’s) in the left-hand column and unclassified particles. In 2B, by manual inspection, WBC casts were identified. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig 4: Histochemical staining of skin lesions. (a) Numerous mast cells are located in suppurative foci. TB stain. (b) Fibre proliferated mildly in infected foci. L: infected foci; Ds: the fibrous tissue of the dermis. Mallory trichrome stain. (c) No fungal hyphae are observed in the infected area. PAS stain. (d) There is no fluorescent signal of acid‐fast bacteria in the infected area. Acid‐fast (fluorescence) stain. a, b and c are captured by Olympus CX43 microscope and EPview Ver 1.2. d is captured by Olympus BX53 fluorescence microscope and cellSens standard Ver 3.1

Fig 5: Number of microplastics in water samples in Lago Menor of Lake Titicaca.Box plot for the microplastic number found in the water samples in different sites in Lago Menor of Lake Titicaca. Visual examination was performed using an Olympus CX43 microscope, 4X and 10X objectives.