G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) from Cytoskeleton, Inc.

Product Specs
ItemG-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S)
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberBK135
Quantity3 KITS (24 assays/kit)
ApplicationsELISA
Detection TargetRhoA
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) Alveolar repair following LPS-induced injury requires cell-ECM interactionsJCI insightJuly 24, 2023Sucre JM, Bock F, Negretti NM, Benjamin JT, Gulleman PM, Dong X, Ferguson KT, Jetter CS, Han W, Liu Y, et al.incubation. Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton catalog BK135), where levels of activated RhoA, Cdc42, and Rac1 were detected colorimetrically per manufacturer’s

(2) Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorptionThe Journal of cell biologyApril 3, 2023Zhu L, Tang Y, Li XY, Kerk SA, Lyssiotis CA, Sun X, Wang Z, Cho JS, Ma J, Weiss SJ.lysates were harvested, and Rho GTPase activity analyzed using RhoA/Rac1 G-LISA Activation Assay kits (BK135; Cytoskeleton) according to the manufacturer’s instructions. Lentiviral or retroviral gene transduction Where

(3) Different hotspot p53 mutants exert distinct phenotypes and predict outcome of colorectal cancer patientsNature CommunicationsDecember 1, 2022Ori Hassin et al.determined by using an enzyme-linked immunosorbent assay (ELISA)-based G-LISA kit (Cytoskeleton, Inc #BK135) strictly following the manufacturer’s instructions. Briefly, SW480 cells stably overexpressing p53R175H

(4) Aberrant activation of TGF-Î²1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann diseaseFront Endocrinol (Lausanne)October 17, 2022Qi Chen, Yan Yao, Kun Chen, Xihui Chen, Bowen Li, Rui Li, Lidangzhi Mo, Weihong Hu, Mengjie Zhang, Zhen Wang, et al.respectively. Rho GTPase activation assay The assay was implemented through a GTPase G-LISA activation assay kit (BK135, Cytoskeleton, Inc.). After washing with PBS, RAW264.7 or HEK293T cells were treated with a cold lysis

(5) Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factorsCell reportsFebruary 22, 2022Nataraj NB, Noronha A, Lee JS, Ghosh S, Mohan Raju HR, Sekar A, Zuckerman B, Lindzen M, Tarcitano E, Srivastava S, et al.LTX and Plus,Invitrogen,Cat# 15338100 Rho/Rac/cdc42 G-LISA activation kit,"Cytoskeleton, Inc",Cat# BK135 3D assay kit,Cultrex,Cat# 3500096K FluoroBlok,Corning,Cat# 351157 Ras activation kit,"Cytoskeleton, Inc

(6) Profilin2 regulates actin rod assembly in neuronal cellsScientific ReportsDecember 1, 2021Lisa Marie Walter, Sebastian Rademacher, Andreas Pich, Peter Clausactivity of small G-proteins (Rac1, RhoA and Cdc42) was measured by utilizing G-LISA Activation Assays (BK135, Cytoskeleton, Denver, Colorado, USA). The assays were performed according to the manufacturer’s instructions.

(7) Cancer-associated mutations in VAV1 trigger variegated signaling outputs and T-cell lymphomagenesisThe EMBO journalNovember 15, 2021Robles-Valero J, Fernández-Nevado L, Lorenzo-Martín LF, Cuadrado M, Fernández-Pisonero I, Rodríguez-Fdez S, Astorga-Simón EN, Abad A, Caloto R, Bustelo XR.extracts were analyzed using G‐LISA Assay Kits according to the manufacturer’s instructions (Cat. No. BK135, Cytoskeleton). Values are represented as the n‐fold change in the experimental sample relative to the

(8) PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFRCell reportsMay 25, 2021Ghosh S, Nataraj NB, Noronha A, Patkar S, Sekar A, Mukherjee S, Winograd-Katz S, Kramarski L, Verma A, Lindzen M, et al.LTX and Plus,Invitrogen,Cat# 15338100 Rho/Rac/cdc42 G-LISA activation kit,"Cytoskeleton, Inc",Cat# BK135 3D assay kit,Cultrex,Cat# 3500096K FluoroBlok,Corning,Cat# 351157 Luciferase kit,Promega,Cat# E1910 RNA

(9) RhoG deficiency abrogates cytotoxicity of human lymphocytes and causes hemophagocytic lymphohistiocytosisBloodApril 15, 2021Artem Kalinichenko et al.(quantification of GTP-bound protein) of Rac1, Cdc42, and RhoA was assessed using G-LISA Kit (Cytoskeleton Inc, BK135) according to manufacturer’s instructions. Alternatively, analysis of Rac1, Cdc42, and RhoA activation

(10) VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinomaNature communicationsSeptember 22, 2020Lorenzo-Martín LF, Fernández-Parejo N, Menacho-Márquez M, Rodríguez-Fdez S, Robles-Valero J, Zumalave S, Fabbiano S, Pascual G, García-Pedrero JM, Abad A, et al.and Cdc42 G-LISA assay kits according to the manufacturer’s instructions (Cytoskeleton, Catalog No. BK135). Microarray experiments Total RNA from the back epidermis of 8-week-old mice of indicated genotypes

(11) Vav2 pharmaco-mimetic mice reveal the therapeutic value and caveats of the catalytic inactivation of a Rho exchange factorOncogeneJuly 9, 2020L. Francisco Lorenzo-Martín, Sonia Rodríguez-Fdez, Salvatore Fabbiano, Antonio Abad, María C. García-Macías, Mercedes Dosil, Myriam Cuadrado, Javier Robles-Valero, Xosé R. Busteloanalyzed using G-LISA assay kits according to the manufacturer’s instructions (Cytoskeleton, Catalog No. BK135). Values are represented as the n-fold change of the experimental sample relative to the activity shown

(12) The Inhibition of Wnt Restrain KRASG12V-Driven Metastasis in Non-Small-Cell Lung CancerCancers (Basel)March 31, 2020Pei-Shan Hung, Ming-Hung Huang, Yuan-Yeh Kuo, James Chih-Hsin YangAssay The activity of RhoA/Rac1/Cdc42 was determined using a RhoA/Rac1/Cdc42 G-LISA Activation Assay kit (BK135) as recommended by the manufacturer (Cytoskeleton, Inc., Denver, CO, USA). Briefly, the samples were

(13) Lysine Acetylation Reshapes the Downstream Signaling Landscape of Vav1 in LymphocytesCellsMarch 4, 2020Sonia Rodríguez-Fdez, Lucía Fernández-Nevado, L. Francisco Lorenzo-Martín, Xosé R. Busteloand analyzed using Rac1 G-LISA® assay kits according to the manufacturer’s instructions (Cat. number BK135, Cytoskeleton). 2.11. Statistical Analyses All the statistical analyses were carried out using GraphPad

(14) AIF1L regulates actomyosin contractility and filopodial extensions in human podocytesPloS oneJanuary 1, 2018Yasuda-Yamahara M, Rogg M, Yamahara K, Maier JI, Huber TB, Schell C.activity was measured using a Cdc42 G-LISA activation assay kit according to manufacturer´s instructions (BK135, Cytoskeleton, USA). AIF1L immunoprecipitation and interaction proteomics SILAC labeling and quantitative

(15) MicroRNA-141 suppresses prostate cancer stem cells and metastasis by targeting a cohort of pro-metastasis genesNature CommunicationsApril 1, 2017Can Liu et al.activation assays as per the manufacturer's instructions (Cytoskeleton, Inc., Denver, CO; catalogue number BK135). RNA-Seq and bioinformatics DU145 and LAPC9 cells were transfected with 30 nM of miR-141 or NC oligos

(16) Cell-Intrinsic Adaptation Arising from Chronic Ablation of a Key Rho GTPase RegulatorDevelopmental CellAugust 30, 2016Berati Cerikan, Ranad Shaheen, Georgina P. Colo, Christine Gläßer, Shoji Hata, Klaus-Peter Knobeloch, Fowzan S. Alkuraya, Reinhard Fässler, Elmar Schiebel

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G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) from Cytoskeleton, Inc.

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