Fig 1: Ca2+-activated ATPases. Quantification of Pi from ATP hydrolysis by Ca2+-activated ATPase in myocardial tissue extracts enriched for myosins is plotted. (A) Left atrium from a donor heart and skeletal muscle extracts are used as technical controls. Representative histochemistry images showing the homogeneous distribution of active fast and slow ATPase on cryosections from HCM and CTRL are shown (B). Notice the absence of difference in the brown signal intensity, irrespective of the presence of disarray in the HCM field. Tissue contents of fast (ATP2A1, (C)) and slow (ATP2A2, (D)) ATPase and their ratio measured by ELISA in extracts from HCM (n = 17) and CTRL (n = 8) are shown (E). Data are presented as boxes (min to max) and dots indicate single sample values. Dark blue dots indicate HCM patients with mutation, light blue dots those without. Mann–Whitney test was applied and significant difference is shown as * p < 0.05.
Supplier Page from MyBioSource.com for Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) ELISA Kit