Fig 1: Concentrations of myo-inositol oxygenase (MIOX) in kidneys of LB (olive) and LSL (navy) hens at five productive periods. Symbols show mean ± SEM (n = 10 hens per strain and period). Two-way ANOVA followed by the post-hoc test Tukey HSD (Honestly Significant Difference) were used for statistical analysis. Different letters indicate significant differences within strain at different productive periods (p < 0.05) whereas **indicate highly significant differences (p < 0.01) between strains at the same productive period. F-values showed the ratio of between-groups to within-groups variances.
Fig 2: Pearson’s correlations using pattern search function in MetaboAnalyst 4.0 between components of MI metabolism and plasma metabolites in all hens and all time points. (a) Correlation to plasma MI; (b) correlation to kidney myo-inositol oxygenase (MIOX); (c) correlation to muscle inositolmonophosphatase 1(IMPase 1); (d) correlation to liver IMPase 1. Correlations with Pearson’s correlation coefficient (r) = 0.4 (*) were discussed in detail in the text; corresponding statistical information is provided in Supplementary Table S1. Ala alanine, Alpha-AAA alpha-aminoadipic acid, Arg arginine, Asn asparagine, Asp aspartate, C0 carnitine, ADMA asymmetric dimethylarginine, Ala alanine, C3 propionylcarnitine, C3:1 propenoylcarnitine, C4 butyrylcarnitine, C10 decanoylcarnitine, C10:1 decenoylcarnitine, C10:2 decadienylcarnitine, C12-DC dodecanedioylcarnitine, C14 tetradecanoylcarnitine, C16:1 hexadecenoylcarnitine, C18:1-OH hydroxyoctadecenoylcarnitine, DOPA dihydroxyphenylalanine, Gly glycine, Glu glutamate, H1 sum of hexoses, IMPase 1 myo-inositol monophosphatse 1, Lyso PC lysophosphatidylcholines, Met methionine, Met-SO Methionine sulfoxide, MI myo-inositol, MIOX myo-inositol oxygenase, PC phosphatidylcholine, SDMA symmetric dimethylarginine, SM sphingomyelins, t4-OH-Pro trans-4-hydroxyproline, Tyr tyrosine.
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