NEBuilder HiFi DNA Assembly Cloning Kit from New England Biolabs

NEBuilder® HiFi DNA Assembly Cloning Kit

Developed to improve the efficiency and accuracy of DNA assembly, NEBuilder HiFi DNA assembly allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. The reaction includes different enzymes that work together in the same buffer:

• The exonuclease creates single-stranded 3´ overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region)
• The polymerase fills in gaps within each annealed fragment
• The DNA ligase seals nicks in the assembled DNA

The end result is a double-stranded fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E coli. NEBuilder HiFi DNA Assembly Cloning Kit is supplied with NEB 5-alpha High Efficiency Competent E.coli.

Features:

• Save time with Simple and Fast Seamless Cloning.
• Use one system for both "standard-size" cloning and larger gene assembly products, up to 6 fragments.
• Move on with your workflow, because DNA can be used immediately for transformation or as template for PCR or RCA.
• Adapts easily for multiple DNA manipulations, including site-directed mutagenesis.
• No licensing fee requirements from NEB for the NEBuilder products.

Product Details:

• NEBuilder® HiFi DNA Assembly Cloning Kit E5520S - 10 rxns
• NEBuilder® HiFi DNA Assembly Master Mix E2621S/L/X - 10/50/250 rxns
• NEBuilder® HiFi DNA Assembly Bundle for Large Fragments E2623S - 20 rxns