Description
Recombinant produced leptin receptor (SOB-R) is immobilized on a microtiter plate. The sample to be tested is diluted in dilution buffer and incubated on the microtiter plate; the leptin bound by the immobilized receptor is detected by a specific, polyclonal, Biotin-conjugated antibody and a streptavidin-conjugate. The quantification is performed with recombinant leptin, which is used as a standard and is traceable to the WHO International Standard (97/594)