Fig 1: Chrysin suppresses the proliferation and promotes apoptosis of ESCC tumors in vivo. (A) Animals harbored the indicated ESCC tumors were treated different doses of chrysin (10, 25, and 50 mg/kg/day, p.o.). The growth curves and representative images of tumor were shown. (B)–(G) Cleaved PARP (B), cleaved caspase 3 (C), and pFAK Tyr397/FAK (D), pAKT Ser473/AKT (E), pPRAS40 Thr246/PRAS40 (F), or pRPS6 Ser235/236/RPS6 (G) ratio in the indicated tumors was evaluated using quantitative ELISA assay. (H) Histopathologic analyses of major organs, including heart, liver, spleen, or kidney from control and different doses of chrysin (10, 25, and 50 mg/kg/day, p.o.). Magnification, 3 mm as indicated. *P < 0.05; **P < 0.01; ***P < 0.001. Error bars, mean ± SD of five independent experiments.