Fig 1: miR-137-3p agomir reduces hepatic oxidative stress and inflammation in HFD mice. (a) Mice were fed with a HFD for 24 weeks to establish NAFLD and were also intraperitoneally injected with the miR-137-3p agomir or agomir control (100 mg/kg weekly) at the last 6 consecutive weeks. Relative hepatic ROS level determined by DCFH-DA probe (n = 6). (b) Relative H2O2 level in the liver (n = 6). (c) MDA generation in the liver (n = 6). (d) NRF2 protein expression determined by western blot (n = 6). (e) GSH level in the liver (n = 6). (f) Total SOD and CAT activity in the liver (n = 6). (g) p65 phosphorylation determined by western blot (n = 6). (h, i) Hepatic IL-1β, IL-6, MCP-1, TNF-α, and IL-10 levels determined by the commercial ELISA kits (n = 6). Data were expressed as the means ± standard deviations, and ∗p < 0.05 was considered significant.
Fig 2: Patient-to-patient variability of cytokine expression in foreskin explants before and after ex vivo IR exposure. Graphic presentation of the quantification of IL-6 (A), IL-8 (B), and MCP-1 (C) measured by ELISA in the supernatant of foreskin explants before (non-IR) and after IR exposure (10 Gy; 24 h post-IR) for individual donors (n = 12). * p < 0.1; ** p < 0.01; *** p < 0.001.
Fig 3: Promoting effect of cytokines secreted from hypoxic NRK-52E cells on the proliferation of macrophage RAW264.7. (A) Influence of oligosaccharides on the proliferation of RAW264.7 cells treated with 0.1 mg/mL FC, FOS, or GOS and cultured overnight. (B) Promoting effect of the culture medium of hypoxic NRK-52E cells on the proliferation of RAW264.7 cells. NRK-52E cells were pretreated with 0.1 mg/mL FC, FOS, or GOS for 30 min and then subjected to hypoxic culture. The culture medium of normal or hypoxic NRK-52E cells was taken out and used to culture RAW264.7 overnight. (C) MCP-1 level in the culture medium of hypoxic NRK-52E cells. (D) IL-1β level in the culture medium of hypoxic NRK-52E cells. Results are expressed as mean ± SD (n = 3).
Fig 4: H2A.J and SA-ß-Gal expression in murine skin before and after fractionated in vivo IR exposure. (A) Graphic presentation of the quantification of H2A.J+ and SA-ß-Gal+ cells in different regions of murine hair follicles (infundibulum, bulge, papilla) before (non-IR) and at different time-points after fractionated in vivo irradiation (72 h, 1 w, and 2 w post-IR). * p < 0.1; ** p < 0.01; *** p < 0.001 (B) Graphic presentation of the quantification of IL-6, IL-8, and MCP-1 measured by ELISA in the supernatant of murine skin before (non-IR) and after fractionated in vivo irradiation (5 × 2Gy; 72 h, 1 w, and 2 w post-IR). Data are presented as mean ± SD (n = 3).
Fig 5: Reducing effect of oligosaccharides on serum creatinine and cytokines in AKI mice at the early stage. Blood was collected from each mouse 2 days after IRI surgery to measure serum creatinine (A), MCP-1 (B), IL-1β (C), and TNF-α (D). The results are expressed as means ± SD (n = 8).
Supplier Page from Abcam for Mouse MCP1 ELISA Kit