Fig 1: Hypoxia-induced ADSC-Exos inhibited fibrous and degenerated cartilage in IL-1β-induced OA-like articular chondrocytes. (A) Fibrous cartilage genes, col1a1, and (B) degeneration marker gene, col10a1, mRNA expression in vitro in HACs treated with IL1-β and with or without ADSC-Exos (109 particles/mL) for 12 days. GAPDH mRNA was used as the internal control. (C) Type I and (D) type X collagen ELISAs were performed to quantify the protein levels when HACs were treated with IL1-β and with or without ADSC-Exos (107~109 particles/mL) for 12 days. Each column represents the mean ± SEM of six replicate cultures. Data from each group were compared with those of the IL-1β only group, *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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