Description
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLAC1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antigen preparation specific for PLAC1 and incubated. The competitive inhibition reaction is launched between with HRP labeled PLAC1 and unlabeled PLAC1 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PLAC1 in the sample. The color development is stopped and the intensity of the color is measured.
Background: Cocchia et al. (2000) isolated a novel gene, which they designated PLAC1, approximately 65 kb telomeric of the HPRT gene. The mouse homolog was also found to map near Hprt on the X chromosome. Human PLAC1 is transcribed in the telomeric to centromeric direction, opposite to the orientation of HPRT, and contains 3 predicted exons. It encodes a deduced 212-amino acid protein that shares 60% sequence identity with mouse Plac1. The human and mouse proteins contain a signal peptide and a ZP3 motif. Northern blot analysis detected placenta-restricted expression of a 1.7-kb PLAC1 mRNA transcript. In situ hybridization studies in mouse detected Plac1 expression from 7.5 to 14.5 days postcoitum in ectoplacental cone, giant cells, and labyrinthine trophoblasts