Fig 1: Enzyme-linked immunosorbent assay was used to determine the protein levels of HR-associated factors. The measurement of the protein levels of Mre11 (a), BRCA1 (b), and Rad51 (c) in both cells treated for 48 hours showed that the levels of BRCA1 was not affected by AZD2461+VPA, while this combination decreased Mre11 and Rad51 levels only in PC-3 cells (**P<0.05).
Fig 2: Primer sequences of PTEN, BRCA1, Rad51, Mre11 and GAPDH
Fig 3: Real-time PCR method was employed to determine the mRNA level of HR-associated genes. Basal mRNA levels of BRCA1, Mre11, Rad51, and PTEN in PC-3 cells compared to DU145 cells indicates that DU145 cells have a higher basal expression of these genes (a). The measured mRNA levels of Mre11 (b), BRCA1 (c), and Rad51 (d) genes in both cells treated for 48 hours with AZD2461, VPA and their combination has revealed that these regimen was only effective in reducing Mre11 and Rad51 expression in PC-3 cells. Each assay was normalized to housekeeping gene GAPDH (**p < 0.05).
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