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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Antigen density quantification of cell-surface immunotherapy targets by flow cytometry: Multi-antigen assay of neuroblastoma bone marrow metastasisSTAR ProtocolsDecember 1, 2023Molly T. Radosevich, Scott J. Bornheimer, Majid Mehrpouryan, Bita Sahaf, Jean S. Oak, Crystal L. Mackall, Sabine HeitzenederConjugation Kit Lightning-Link,Abcam,Cat# ab102918 Abcam APC Conjugation Kit Lightning-Link,Abcam,Cat# ab201807 BD Quantibrite PE beads,BD Biosciences,Cat# 340495 BD custom quantitation beads in APC,BD Biosciences,Custom

(2) Local analgesia of electroacupuncture is mediated by the recruitment of neutrophils and released β-endorphinsPainSeptember 1, 2023Jing-tao Shi, Wan-ying Cao, Xiao-Ning Zhang, Hong-Ye Wan, Yang-Shuai Su, Zheng-Yang Qu, Rui Wang, Wei He, Xiang-Hong Jing, Xiao-Yu Wangwere incubated in the dark with the following antibodies: anti-β-END antibodies conjugated with APC (ab201807, Abcam), PE Mouse anti-Rat CD11b (562105, BD Pharmingen, New Jersey), and anti-ICAM1 antibodies conjugated

(3) Dual targeting ovarian cancer by Muc16 CAR-T cells secreting a bispecific T cell engager antibody for an intracellular tumor antigen WT1Research SquareMay 9, 2023Sung Soo Mun, Leila Peraro, Jeremy Meyerberg, Tatyana Korontsvit, Manish Malviya, Thomas Gardner, Chrisann Kyi, Roisin E. O’Cearbhaill, Cheng Liu, Tao Dao, et al.(Emeryville, CA), and APC conjugation was done according to the instructions of the manufacturer (Abcam, ab201807). Mab against Muc16 (clone 4H11) was a kind gift from Dr. Renier Brentjens. Mab against human HLA A*02

(4) The CTLA‐4 immune checkpoint protein regulates PD‐L1:PD‐1 interaction via transendocytosis of its ligand CD80The EMBO JournalMarch 1, 2023Alan Kennedy, Maximillian A Robinson, Claudia Hinze, Erin Waters, Cayman Williams, Neil Halliday, Simon Dovedi, David M Sansomgenerated using the APC Conjugation Kit—Lightning‐Link® according to manufacturer's instructions (Abcam, ab201807). APC‐conjugated anti‐PD‐L1 antibodies were procured from ThermoFisher (clone MIH1 [#14‐5983‐82]), or

(5) GPR97 triggers inflammatory processes in human neutrophils via a macromolecular complex upstream of PAR2 activationNature communicationsOctober 27, 2022Chu TY, Zheng-Gérard C, Huang KY, Chang YC, Chen YW, I KY, Lo YL, Chiang NY, Chen HY, Stacey M, et al.(130-126-529, Miltenyi Biotec), anti-CD66b-PerCP-Cy5.5 (305108, Biolegend), and G97-A conjugated with APC (Ab201807, Abcam) for 20 min. Fluorescent minus one controls include GPR97 and aqua fluorescent reactive dye only

(6) Immune Enhancement of Nanoparticle-Encapsulated Ginseng Stem-Leaf Saponins on Porcine Epidemic Diarrhea Virus Vaccine in MiceVaccines (Basel)October 27, 2022Fei Su, Lihua Xu, Yin Xue, Wei Xu, Junxing Li, Bin Yu, Shiyi Ye, Xiufang YuanPEDV nucleocapsid protein was labeled with the fluorescent dye APC by using an APC conjugation kit (ab201807, Abcam, Shanghai, China). The relative fluorescence intensity of intracellular PEDV was quantified on

(7) Impact of CTLA-4 checkpoint antibodies on ligand binding and TransendocytosisFrontiers in ImmunologyAugust 31, 2022Cayman Williams, Alan Kennedy, Maximillian A. Robinson, Christopher Lloyd, Simon J. Dovedi, David M. Sansompharmacy. Fluorochrome conjugation was carried out using the PE/R-Phycoerythrin (Cat# ab102918) or APC (Cat# ab201807) conjugation kit - Lightning-link (Abcam) according to manufacturers instructions and the protein used

(8) Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanismImmunityJuly 12, 2022Gregoire C, Spinelli L, Villazala-Merino S, Gil L, Holgado MP, Moussa M, Dong C, Zarubica A, Fallet M, Navarro JM, et al.PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®,Abcam,Cat# ab102918 APC Conjugation Kit - Lightning-Link®,Abcam,Cat# ab201807 Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link®,Abcam,Cat# ab269824 Tamoxifen,Fisher Scientific,Cat#

(9) Workflow Optimization for Identification of Female Germline or Oogonial Stem Cells in Human Ovarian Cortex Using Single-Cell RNA Sequence AnalysisStem CellsMay 27, 2022Hannah Alberico, Zoë Fleischmann, Tyler Bobbitt, Yasushi Takai, Osamu Ishihara, Hiroyuki Seki, Richard A Anderson, Evelyn E Telfer, Dori C Woods, Jonathan L Tilly(MA3100, 1:50; Invitrogen–ThermoFisher Scientific, Waltham, MA), each directly conjugated to APC (Abcam, ab201807), were used for determination of the total percentage of autofluorescent events that were positive for

(10) Rationally targeted anti-VISTA antibody that blockades the C-C' loop region can reverse VISTA immune suppression and remodel the immune microenvironment to potently inhibit tumor growth in an Fc independent mannerJournal for immunotherapy of cancerFebruary 1, 2022Thakkar D, Paliwal S, Dharmadhikari B, Guan S, Liu L, Kar S, Tulsian NK, Gruber JJ, DiMascio L, Paszkiewicz KH, et al.#14-9165-42) prior to staining. HMBD-002 or isotype control antibodies were conjugated with APC (Abcam # ab201807) as per manufacturers protocol. For fluorescence activated cell sorting (FACS), cells were incubated

(11) Dysregulation of the CD163-Haptoglobin Axis in the Airways of COPD PatientsCellsDecember 21, 2021Higham A, Baker JM, Jackson N, Shah R, Lea S, Singh D.antibodies: haptoglobin (antibodies-online, ABIN952678 (primary), APC Conjugation Kit (secondary), Abcam, ab201807), CD163 (Thermo Fisher Scientific, Loughborough, UK 12-1639-42), CD206 (Biolegend, London, UK 321120)

(12) CD8⁺ T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuityImmunityMay 11, 2021Nguyen THO, Rowntree LC, Petersen J, Chua BY, Hensen L, Kedzierski L, van de Sandt CE, Chaurasia P, Tan HX, Habel JR, et al.AB_2868861" Streptavidin PE,Thermo Fisher Scientific,Cat#S866 APC Conjugation Lightning-Link Kit,Abcam,Cat#ab201807 "Peroxidase AffiniPure goat anti-human IgG, Fcγ fragment specific",Jackson ImmunoResearch,Cat#109-035-098;

(13) Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomesOncology lettersJanuary 1, 2021Fujimoto T, Tsunedomi R, Matsukuma S, Yoshimura K, Oga A, Fujiwara N, Fujiwara Y, Matsui H, Shindo Y, Tokumitsu Y, et al.cognate isotype control antibodies were conjugated with APC using an APC conjugation kit (cat. no. ab201807; Abcam) according to the manufacturer's instructions. Rat IgG2a κ Isotype Control (cat. no. 14‑4321‑82;

(14) Pre-existing immunity to influenza virus hemagglutinin stalk might drive selection for antibody-escape mutant viruses in a human challenge modelNature MedicineAugust 1, 2020Jae-Keun Park et al.Millipore Sigma) was conjugated with allophycocyanin (APC) using an APC Conjugation Kit (catalog no. Ab201807, Abcam) and used for gating. The estimated concentrations of the conjugated antibodies were 0.83 mg

(15) Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression MappingCell stem cellOctober 3, 2019Severe N, Karabacak NM, Gustafsson K, Baryawno N, Courties G, Kfoury Y, Kokkaliaris KD, Rhee C, Lee D, Scadden EW, et al.the NGFR purified antibody (Lifespan Biosciences, LS-C179536) using the APC conjugation kit (Abcam, ab201807) according to the manufacturer instructions. Peripheral blood analysis To assess the regeneration

(16) CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cellsPloS oneJanuary 1, 2019Haque NS, Tuteja A, Haque N.Anti-CCR8 was generated as described (10). Anti-CCR8 was conjugated using the APC Conjugation Kit (ab201807) which purchased from Abcam. Antibodies against MSC markers PDGFRα (clone αR1) and Purified Mouse Anti-Rat Nestin

(17) Targeted Imaging of Renal Fibrosis Using Antibody-Conjugated Gold Nanoparticles in Renal Artery StenosisInvestigative RadiologyOctober 1, 2018Xiang-Yang Zhu, Xiangyu Zou, Rahul Mukherjee, Zhicong Yu, Christopher M. Ferguson, Wei Zhou, Cynthia H. McCollough, Lilach O. Lermanprotocol, except that collagen-I antibody was pre-conjugated with APC using an APC conjugation kit (Abcam ab201807), following the provided protocol. 2. Collagen binding tests of Co-I-AuNPs The rationale for tests

(18) Single-Cell RNA-seq Reveals a Subpopulation of Prostate Cancer Cells with Enhanced Cell-Cycle–Related Transcription and Attenuated Androgen ResponseCancer ResearchFebruary 15, 2018Aaron M. Horning et al.were incubated with anti-CD81 PE-conjugated (ab81436, AbCam) and anti-HMMR APC-conjugated (ab170527 & ab201807, AbCam). The HMMRhi (8.1) cells were sorted using Beckman Coulter Moflow Astrios. In vitro phenotypic

(19) Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancerCell researchApril 1, 2017Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, et al.17-1278). PITPNM3 antibody (Cat# NBP1-31070, Novus) was labeled with APC by Abcam APC Conjugation Kit (ab201807, Abcam) according to the manufacturer's instructions. For the intracellular stain, cells were pretreated

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APC Conjugation Kit - Lightning-Link from Abcam

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APC Conjugation Kit - Lightning-Link from Abcam

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