Fig 1: Mucin 2 and mucin 3 quantification in HT29-16E cells after treatment with seven Bacillus spp. strains. HT29-16E cells were incubated with the seven Bacillus spp. strains for 10 h. The cells’ supernatants were taken and mucin 2 (MUC2) production was measured in this fraction by ELISA. The cells were then detached with trypsin, collected and lysed by ultrasonication, and mucin 3 (MUC3) was measured in this cell fraction by ELISA. Data are expressed as mean ± SEM of two independent experiments performed in duplicates. Two-way ANOVA and multiple comparison tests were performed to assess statistically significant differences between control and different probiotic treatments for MUC2 and MUC3, respectively. p-value = 0.0001 (****).