Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay N1620 from Promega

Product Specs
ItemNano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1620
MethodLuciferase
Sample TypeAssays or screens in any plate size
Detection TargetLuciferase
ReactivityMammalian
Quantity100 ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTDNature communicationsSeptember 11, 2023Latallo MJ, Wang S, Dong D, Nelson B, Livingston NM, Wu R, Zhao N, Stasevich TJ, Bassik MC, Sun S, et al.after the second transfection. Dual-luciferase assay Nano-Glo Dual Luciferase Assay system (Promega, N1620) was used to measure the NLuc and FLuc luciferase activities in cells transfected with frameshift reporters

(2) The ribosome-inactivating proteins MAP30 and Momordin inhibit SARS-CoV-2PLOS ONEJune 29, 2023Norman R. Watts, Elif Eren, Ira Palmer, Paul L. Huang, Philip Lin Huang, Robert H. Shoemaker, Sylvia Lee-Huang, Paul T. Wingfieldcompound). After incubating plates at 37°C/5% CO2 and 90% humidity for 72 hours, 30 μL of NanoGlo (Promega #N1620) was added to each well according to the manufacturer’s instructions with the following modifications:

(3) Activation of Gcn2 by small molecules designed to be inhibitorsJournal of Biological ChemistryApril 1, 2023Kenneth R. Carlson, Millie M. Georgiadis, Feven Tameire, Kirk A. Staschke, Ronald C. Weknano-luciferase signals were measured with Nano-Glo Dual-Luciferase Reporter Assay kit (Promega Cat #N1620) using a Turner Biosystems 20/20 luminometer. Firefly luciferase activity was normalized to nano-luciferase

(4) A High-Throughput Amenable Dual Luciferase System for Measuring Toxoplasma gondii Bradyzoite Viability after Drug TreatmentAnalytical ChemistryJanuary 17, 2023David Smith, Matteo Lunghi, Einar B. Olafsson, Olivia Hatton, Manlio Di Cristina, Vern B. Carruthersmeasured at set time points using a Nano-Glo Dual-Luciferase Reporter Assay System (Promega, cat. no. N1620), following the manufacturer’s instructions (outlined in Figure 3A, detailed protocol in the Supporting

(5) A Tead1-Apelin axis directs paracrine communication from myogenic to endothelial cells in skeletal muscleiScienceJuly 15, 2022Umji Lee et al.promoter conjugated with firefly luciferase. Stop&Glo reagents were purchased from Promega (Cat# E8120, N1620, E2661). Three days after transfection, cells were washed with PBS and lysed. After 15 min of incubation,

(6) Comparative analysis of dCas9-VP64 variants and multiplexed guide RNAs mediating CRISPR activationPLOS ONEJune 28, 2022Kohei Omachi, Jeffrey H. Mineradded, and firefly luciferase activity was measured. Subsequently, NanoDLR Stop & Go reagent (Promega, #N1620) was added, and the reaction plate was incubated for 10 min at room temperature, then NanoLuc luciferase

(7) Blimp-1 molds the epigenetic architecture of IL-21-mediated autoimmune diseases through an autoregulatory circuitJCI insightJune 8, 2022Liu YW, Fu SH, Chien MW, Hsu CY, Lin MH, Dong JL, Lu RJ, Lee YJ, Chen PY, Wang CH, et al.were performed 24 hours posttransfection using the Nano-Glo Dual Luciferase Reporter Assay system (N1620, Promega). ChIP assay. CD4+ T cells were cross-linked for 10 minutes by adding formaldehyde to tissue culture medium

(8) NanoLuc reporters identify COL4A5 nonsense mutations susceptible to drug-induced stop codon readthroughiScienceMarch 18, 2022Kohei Omachi, Hirofumi Kai, Michel Roberge, Jeffrey H. Minerassays,Critical commercial assays ,, Nano-Glo® Dual-Luciferase Reporter Assay System,Promega,Cat# N1620 SuperSignal West Pico Chemiluminescent Substrate,Thermo Scientific,Cat# 34580 Amersham ECL select Western Blotting Detection Reagent

(9) Characterization of the endogenous DAF-12 ligand and its use as an anthelmintic agent in Strongyloides stercoraliseLifeDecember 7, 2021Zhu Wang, Mi Cheong Cheong, Jet Tsien, Heping Deng, Tian Qin, Jonathan DC Stoltzfus, Tegegn G Jaleta, Xinshe Li, James B Lok, Steven A Kliewer, et al.reagent,pPV540(plasmid),"Lok, 2019 (PMID:31379923)",N/A, Commercial assay or kit,Nano-Glo Dual-luciferase kits,Promega,N1620, Commercial assay or kit,NADPH regeneration system,Promega,V9510, Commercial assay or kit,Microsome Isolation Kit

(10) FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progressionClinical and translational medicineApril 1, 2021Miao L, Zhuo Z, Tang J, Huang X, Liu J, Wang HY, Xia H, He J.#3420; Cell Signaling Technology) were used as negative controls. For luciferase reporter assays (#N1620; Promega, WI, USA), firefly and Renilla Luciferase activities were examined by the Dual‐Luciferase Reporter Assay System

(11) UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunctionCell ReportsMarch 30, 2021Benjamin L. Zaepfel, Zhe Zhang, Kirstin Maulding, Alyssa N. Coyne, Weiwei Cheng, Lindsey R. Hayes, Thomas E. Lloyd, Shuying Sun, Jeffrey D. RothsteinScientific,Hs00208049_m1 XPNPEP1 Taqman Probe,Thermo Fisher Scientific,Hs00958021_m1 Nano-Glo Dual Luciferase Assay,Promega,Cat#N1620 Experimental models: Cell lines,, Human: HeLa Flp-In dual luciferase reporter cells,"Cheng et al.,

(12) Control of Slc7a5 sensitivity by the voltage-sensing domain of Kv1 channelseLifeNovember 9, 2020Lamothe SM, Sharmin N, Silver G, Satou M, Hao Y, Tateno T, Baronas VA, Kurata HT.,Superscript IV First-strand synthesis system,Invitrogen,18091050, "Commercial assay, kit",Nano-Glo live cell assay reagent,Promega,N1620, "Chemical compound, drug",Puromycin,Gibco,Ref #: A11138-03, "Chemical compound, drug",Polybrene,Sigma Aldrich

(13) CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG TranslationNeuronDecember 4, 2019Cheng W, Wang S, Zhang Z, Morgens DW, Hayes LR, Lee S, Portz B, Xie Y, Nguyen BV, Haney MS, et al.NLuc and FLuc luciferase activities were measured blindly by Nano-Glo Dual Luciferase Assay (Promega, N1620) on Tecan Infinite 200 PRO. NLuc levels were normalized to FLuc, or both were normalized to total protein

(14) Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector ComplexJournal of Molecular NeuroscienceFebruary 1, 2018Ci-Di Chen, Ella Zeldich, Yuexuan Li, Andrea Yuste, Carmela R. Abrahamcoincidence reporter vector under Klotho promoter, Nano-Glo® Dual-Luciferase® Reporter Assay System (cat. N1620, Promega) was used according to manufacturer’s instructions. Briefly, 24 hours after transfection in

(15) Quantitative High‐Throughput Screening Using a Coincidence Reporter BiocircuitCurrent Protocols in NeuroscienceApril 10, 2017Brittany W. Schuck, Ryan MacArthur, James IngleseRE/promoter chosen Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System (Promega catalog no. N1610 or N1620) Graphing software (GraphPad Prism, etc.) Tissue culture hood 37°C, 5% CO2, 95% humidity incubator

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Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay

Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay from Promega

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