Fig 1: Protein expression in tear film before and after Femto-LASIK. (A) Evaluation of MMP-9 values pre-OP as well as 5 and 90 days after Femto-LASIK revealed no significant differences. (B) The NGF level at all three investigation points was nearly the same. (C) The amount of the pro-inflammatory cytokine IL1-β was not altered following Femto-LASIK. (D) The same levels for the pro-inflammatory chemokine IL-8 were identified in all samples. (E) The CGRP level at 5 days post-OP was significantly downregulated in comparison to pre-OP, but not in comparison to 90 days post-OP. Values are mean ± SEM ± SD; * p < 0.05. Abbreviations: CGRP = calcitonin gene-related peptide; IL = interleukin; MMP-9 = matrix metalloproteinase-9; NGF = nerve growth factor.
Fig 2: (A,B) Nestin and NeuN immunofluorescence staining showed changes in cell morphology on days 4 and 7; (C) Neurites’ number, mean length, and total length analysis, n = 8; (D) ELISA results for the expression of BDNF and NGF in pg/mL. Note (*: p < 0.05/**: p < 0.001).
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