Fig 1: Localization of protein S (PROS1). Localization of PROS1 mock, wild type (PROS1-WT), and the p.Leu607Ser mutation (PROS1-M) in HEK293T cells, as detected by immunofluorescence. There is no difference before and after introduction of the PROS1 1820T > C mutation. PROS1 expression was obviously downregulated after mutation
Fig 2: Relative protein S (PROS1) mRNA and protein expression. (a) Relative green fluorescent protein (GFP) expression of cells transfected with mock, wild type (PROS1-WT), and the p.Leu607Ser mutation (PROS1-M) shows that there is no significant difference in transfection efficiency between the three groups. (b) Relative PROS1 mRNA expression of mock, PROS1-WT, and PROS1-M in HEK293T cells with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal reference. (c) Relative PROS1 mRNA expression of mock, PROS1-WT, and PROS1-M in HEK293T cells using GFP as an external reference. (d) PROS1 expression of mock, PROS1-WT, and PROS1-M in the supernatant and lysate of HEK293T cells, as detected by western blot. (e) According to the enzyme-linked immunosorbent assay (ELISA) standard curve, the expression of PROS1 mock, PROS1-WT, and PROS1-M in the supernatant of HEK293T cells is calculated. (f) According to the ELISA standard curve, the expression of PROS1 mock, PROS1-WT, and PROS1-M in HEK293T cell lysate is calculated. *p<0.05,** p<0.01,*** p<0.005, n = 3
Fig 3: Protein S (PROS1) homology modeling and analysis. a Homology modeling of PROS1 has been performed using Swiss-Model. Leu607 is labeled in the alpha helix. b Conserved analysis of amino acid sequences near Leu607 (marked with red) (https://swissmodel.expasy.org/repository/uniprot/P07225?template=1h30.1.A&range=266-673)
Fig 4: Family map. In the family map of hereditary protein S deficiency, the proband (III5) and other patients carry a c. 1820T > C (p.Leu607Ser) PROS1 heterozygous mutation
Supplier Page from Abcam for Human Protein S ELISA Kit