ChargeSwitch PCR Clean-Up Kit CS12000 from Thermo Fisher Scientific

Product Specs
ItemChargeSwitch PCR Clean-Up Kit
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberCS12000
FormatKit
Detection TargetDNA from Enzymatic Reactions (e.g. PCR)
Brand/TrademarkInvitrogen™
Quantity100 Preps
TypePCR Clean-Up Kit
ApplicationsNext-Generation Sequencing (NGS), Cloning, Standard PCR
Sample TypePCR Products
Assay Time5 min

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Diversity-oriented synthesis encoded by deoxyoligonucleotidesNat CommunAugust 15, 2023Liam Hudson, Jeremy W. Mason, Matthias V. Westphal, Matthieu J. R. Richter, Jonathan R. Thielman, Bruce K. Hua, Christopher J. Gerry, Guoqin Xia, Heather L. Osswald, John M. Knapp, et al.B7204S) per sample (37 °C, 1 h) and cleaned up using the ChargeSwitch PCR Clean-Up Kit (Thermo Scientific CS12000) (Supplementary Fig. 21). Assay details Carbonic anhydrase Carbonic anhydrase II (CAII) inhibition

(2) An optimized 4C-seq protocol based on cistrome and epigenome data in the mouse RAW264.7 macrophage cell lineSTAR ProtocolsJune 1, 2022Zhiqiang Huang, Cheng Wang, Eckardt Treuter, Rongrong Fan117590600001 QIAquick PCR purification kit,QIAGEN,Cat# 28104 ChargeSwitch PCR Clean-Up Kit,Thermo Fisher,Cat# CS12000 Qubit Fluorometric Quantification kit,Thermo Fisher,Cat# Q33238 SMARTer DNA unique dual index kits,Takara,Cat#

(3) Integrated exome and RNA sequencing of TFE3-translocation renal cell carcinomaNature CommunicationsSeptember 6, 2021Guangxi Sun, Junru Chen, Jiayu Liang, Xiaoxue Yin, Mengni Zhang, Jin Yao, Ning He, Cameron M. Armstrong, Linmao Zheng, Xingming Zhang, et al.products were separated by electrophoresis in agarose gels, purified with ChargeSwitch™ PCR Clean-Up Kit (CS12000, Invitrogen, Oberhausen, GER) and then sequenced by ABI 3730XL automatic sequencer (Life Technologies). Total

(4) Prevalence and distribution of multilocus sequence types of Staphylococcus aureus isolated from bulk tank milk and cows with mastitis in PennsylvaniaPLOS ONEMarch 12, 2021Asha Thomas, Shubhada Chothe, Maurice Byukusenge, Tammy Mathews, Traci Pierre, Subhashinie Kariyawasam, Erin Luley, Suresh Kuchipudi, Bhushan JayaraoyqiL) (S1 Table). The PCR products were purified using ChargeSwitch® PCR Clean-Up Kit (Invitrogen, CS12000) and sequenced at Penn State Genomics Core Facility. The sequence chromatograms were analyzed for quality

(5) Prospective virome analyses in young children at increased genetic risk for type 1 diabetesNature MedicineDecember 1, 2019Kendra Vehik et al.Waltham, MA) and random hexamers. After short molecule and random hexamer removal with ChargeSwitch (Cat # CS12000, Thermo Fisher, Waltham, MA), molecules were amplified and tagged with a 12 base-pair barcode tag containing

(6) Multi-omics of the gut microbial ecosystem in inflammatory bowel diseasesNatureMay 29, 2019Jason Lloyd-Price et al.Thermo Fisher) and random hexamers. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct56 using AccuPrimeTM

(7) Antimicrobial properties of basidiomycota macrofungi to Mycobacterium abscessus isolated from patients with cystic fibrosisInternational Journal of MycobacteriologyMarch 13, 2019Beverley Cherie Millar, BeverleyCherie Millar, Rachel E Nelson, Juluri R Moore, JuluriR Moore10 min, 72°C.[7] All PCR products were purified using the Chargeswitch® PCR Clean‑Up Kit (Invitrogen CS12000 http://products.invitrogen. com/ivgn/product/CS12000). The cleaned up putative PCR products were sequenced

(8) Expansion of Bacteriophages Is Linked to Aggravated Intestinal Inflammation and ColitisCell host & microbeFebruary 13, 2019Gogokhia L, Buhrke K, Bell R, Hoffman B, Brown DG, Hanke-Gogokhia C, Ajami NJ, Wong MC, Ghazaryan A, Valentine JF, et al.IgA Mouse ELISA Kit,Invitrogen,Cat# EMIGA MagMax Viral RNA Isolation Kit,Thermo Fisher,Cat # AM1939 ChargeSwitch,Thermo Fisher,Cat# CS12000 AccuPrime™ Taq DNA Polymerase System,Thermo Fisher,Cat# 12339016 SuperScript II RT,Thermo Fisher,Cat#

(9) Genomic landscape of oxidative DNA damage and repair reveals regioselective protection from mutagenesisGenome BiologyDecember 7, 2018Anna R. Poetsch, Simon J. Boulton, Nicholas M. LuscombeEDTA, pH8. Samples were purified using a ChargeSwitch PCR Clean-up Kit (Invitrogen, catalog number CS12000). Half of the sample (up to 5 pmol) was saved as input. The other half was processed for pulldown using

(10) Genome-wide profiling of heritable and de novo STR variationsNature MethodsJune 1, 2017Thomas Willems, Dina Zielinski, Jie Yuan, Assaf Gordon, Melissa Gymrek, Yaniv ErlichAmplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). Plasmids were transformed into 50ul of chemically

(11) Impact of Land Use on Arbuscular Mycorrhizal Fungal Communities in Rural CanadaApplied and Environmental MicrobiologyNovember 1, 2013Mulan Dai, Luke D. Bainard, Chantal Hamel, Yantai Gan, Derek LynchThe three PCR products were pooled and purified with a ChargeSwitch PCR clean-up kit (catalogue no. CS12000; Invitrogen). Purified PCR amplicons were normalized at 25 ng ␮l⫺1 with a Savant DNA 120 SpeedVac concentrator

(12) Phylogeography and historical demography of the Pacific Sierra mackerel (Scomberomorus sierra) in the Eastern PacificBMC GeneticsDecember 1, 2010Mónica Domínguez López, Manuel Uribe Alcocer, Píndaro Díaz JaimesQIAquick purification kit (QIAgen No. Cat. 28104) or the ChargeSwitch PCR Clean-Up kit (Invitrogen, Cat. CS12000). Purified products were sequenced in the forward direction on an ABI 3730xl automated sequencer applying

(13) Making Temperature-Sensitive MutantsMethods in EnzymologyOctober 16, 2010Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke, Philip Hietercells via electroporation. Purify the PCR product using the ChargeSwitch® Clean-up Kit (Invitrogen Cat #CS12000). Set up a ligation reaction that includes the following components: 0.5μl SB221 TOPO-TA, 1.0 μl ligation

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ChargeSwitch PCR Clean-Up Kit from Thermo Fisher Scientific

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ChargeSwitch PCR Clean-Up Kit from Thermo Fisher Scientific

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